The θ Subunit of Escherichia coli DNA Polymerase III: a Role in Stabilizing the ε Proofreading Subunit

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   FIG. 1.

   Effect of holE on the Rif^r mutant frequencies of dnaQ49 strains. Mutant frequencies were determined as described in Materials and Methods from 15 independent cultures grown at the indicated temperatures. The strains used were KA796 (wild type) and NR11852 (ΔholE) and their respective dnaQ49 transductants, constructed as indicated in Materials and Methods. Note the log scale on the y axis. The frequencies for the two dnaQ⁺ strains (hol⁺ and ΔholE) were similar in this experiment, and the data points overlap (line indicated by dnaQ⁺).