Modulation of Deoxysugar Transfer by the Elloramycin Glycosyltransferase ElmGT through Site-Directed Mutagenesis▿
ABSTRACT
The glycosyltransferase ElmGT from Streptomyces olivaceus is involved in the biosynthesis of the antitumor drug elloramycin, and it has been shown to possess a broad deoxysugar recognition pattern, being able to transfer different l- and d-deoxysugars to 8-demethyl-tetracenomycin C, the elloramycin aglycone. Site-directed mutagenesis in residues L309 and N312, located in the α/β/α motif within the nucleoside diphosphate-sugar binding region, can be used to modulate the substrate flexibility of ElmGT, making it more precise for transfer of specific deoxysugars.
FOOTNOTES
- Received 15 December 2008.
- Accepted 11 February 2009.
- ↵*Corresponding author. Mailing address: Departamento de Biología Funcional e Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Universidad de Oviedo, 33006 Oviedo, Spain. Phone and fax: 34 985 103652. E-mail: jasalas{at}uniovi.es
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↵† Present address: Instituto de Biotecnología de León (INBIOTEC), Parque Científico de León, 24006 León, Spain.
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↵▿ Published ahead of print on 20 February 2009.
- American Society for Microbiology
