Cover photograph (Copyright © 2006, American Society for Microbiology. All Rights Reserved.): To characterize the cell cycle timing of chromosome segregation in Caulobacter crescentus, the intracellular location of the origin-proximal part of the chromosome was characterized. Shown is an overlay image of cytoplasmic membrane staining using the red fluorescent dye FM4-64 and the intracellular location of the chromosomal origin visualized using a cyan fluorescent protein-LacI protein fusion binding to a lac operator cassette. The chromosome is oriented with the origin-proximal region located at a cell pole, and one of the newly replicated origins moves to the opposite pole of the cell immediately after initiation of DNA replication. (See related article on page 2244.)