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Cover photograph (Copyright © 2007, American Society for Microbiology. All Rights Reserved.): Three-dimensional reconstruction of z-stack images from a frozen section of a 21-day biofilm formed in the chinchilla middle ear by nontypeable Haemophilus influenzae and labeled for type IV pilin protein (green fluorescence) and with DAPI for detection of double-stranded DNA (dsDNA) (blue fluorescence). DAPI labeling of dsDNA that has formed a dense interwoven meshwork within the biofilm is evident. Pilin protein, or possibly bacteria expressing type IV pili, is seen as small aggregates in a plane that sits slightly below the meshwork of dsDNA strands. (See related article on page 3868.)
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