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Research Article

An alkyl hydroperoxide reductase induced by oxidative stress in Salmonella typhimurium and Escherichia coli: genetic characterization and cloning of ahp.

G Storz, F S Jacobson, L A Tartaglia, R W Morgan, L A Silveira, B N Ames
G Storz
Department of Biochemistry, University of California, Berkeley 94720.
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F S Jacobson
Department of Biochemistry, University of California, Berkeley 94720.
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L A Tartaglia
Department of Biochemistry, University of California, Berkeley 94720.
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R W Morgan
Department of Biochemistry, University of California, Berkeley 94720.
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L A Silveira
Department of Biochemistry, University of California, Berkeley 94720.
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B N Ames
Department of Biochemistry, University of California, Berkeley 94720.
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DOI: 10.1128/jb.171.4.2049-2055.1989
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ABSTRACT

The ahp genes encoding the two proteins (F52a and C22) that make up an alkyl hydroperoxide reductase were mapped and cloned from Salmonella typhimurium and Escherichia coli. Two classes of oxidant-resistant ahp mutants which overexpress the two proteins were isolated. ahp-1 was isolated in a wild-type background and is dependent on oxyR, a positive regulator of defenses against oxidative stress. ahp-2 was isolated in an oxyR deletion background and is oxyR independent. Transposons linked to ahp-1 and ahp-2 or inserted in ahp mapped the genes to 13 min on the S. typhimurium chromosome, 59% linked to ent. Deletions of ahp obtained in both S. typhimurium and E. coli resulted in hypersensitivity to killing by cumene hydroperoxide (an alkyl hydroperoxide) and elimination of the proteins F52a and C22 from two-dimensional gels and immunoblots. ahp clones isolated from both S. typhimurium and E. coli complemented the cumene hydroperoxide sensitivity of the ahp deletion strains and restored expression of the F52a and C22 proteins. A cis-acting element required for oxyR-dependent, rpoH-independent heat shock induction of the F52a protein was present at the S. typhimurium but not the E. coli ahp locus.

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An alkyl hydroperoxide reductase induced by oxidative stress in Salmonella typhimurium and Escherichia coli: genetic characterization and cloning of ahp.
G Storz, F S Jacobson, L A Tartaglia, R W Morgan, L A Silveira, B N Ames
Journal of Bacteriology Apr 1989, 171 (4) 2049-2055; DOI: 10.1128/jb.171.4.2049-2055.1989

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An alkyl hydroperoxide reductase induced by oxidative stress in Salmonella typhimurium and Escherichia coli: genetic characterization and cloning of ahp.
G Storz, F S Jacobson, L A Tartaglia, R W Morgan, L A Silveira, B N Ames
Journal of Bacteriology Apr 1989, 171 (4) 2049-2055; DOI: 10.1128/jb.171.4.2049-2055.1989
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