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Research Article

Pseudomonas aeruginosa outer membrane lipoprotein I gene: molecular cloning, sequence, and expression in Escherichia coli.

M Duchêne, C Barron, A Schweizer, B U von Specht, H Domdey
M Duchêne
Laboratorium für Molekulare Biologie, Ludwig-Maximilians-Universität München, Federal Republic of Germany.
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C Barron
Laboratorium für Molekulare Biologie, Ludwig-Maximilians-Universität München, Federal Republic of Germany.
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A Schweizer
Laboratorium für Molekulare Biologie, Ludwig-Maximilians-Universität München, Federal Republic of Germany.
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B U von Specht
Laboratorium für Molekulare Biologie, Ludwig-Maximilians-Universität München, Federal Republic of Germany.
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H Domdey
Laboratorium für Molekulare Biologie, Ludwig-Maximilians-Universität München, Federal Republic of Germany.
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DOI: 10.1128/jb.171.8.4130-4137.1989
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ABSTRACT

Lipoprotein I (OprI) is one of the major proteins of the outer membrane of Pseudomonas aeruginosa. Like porin protein F (OprF), it is a vaccine candidate because it antigenically cross-reacts with all serotype strains of the International Antigenic Typing Scheme. Since lipoprotein I was expressed in Escherichia coli under the control of its own promoter, we were able to isolate the gene by screening a lambda EMBL3 phage library with a mouse monoclonal antibody directed against lipoprotein I. The monocistronic OprI mRNA encodes a precursor protein of 83 amino acid residues including a signal peptide of 19 residues. The mature protein has a molecular weight of 6,950, not including bound glycerol and lipid. Although the amino acid sequences of protein I of P. aeruginosa and Braun's lipoprotein of E. coli differ considerably (only 30.1% identical amino acid residues), peptidoglycan in E. coli, are identical. Using lipoprotein I expressed in E. coli, it can now be tested whether this protein alone, without P. aeruginosa lipopolysaccharide contaminations, has a protective effect against P. aeruginosa infections.

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Pseudomonas aeruginosa outer membrane lipoprotein I gene: molecular cloning, sequence, and expression in Escherichia coli.
M Duchêne, C Barron, A Schweizer, B U von Specht, H Domdey
Journal of Bacteriology Aug 1989, 171 (8) 4130-4137; DOI: 10.1128/jb.171.8.4130-4137.1989

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Pseudomonas aeruginosa outer membrane lipoprotein I gene: molecular cloning, sequence, and expression in Escherichia coli.
M Duchêne, C Barron, A Schweizer, B U von Specht, H Domdey
Journal of Bacteriology Aug 1989, 171 (8) 4130-4137; DOI: 10.1128/jb.171.8.4130-4137.1989
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