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Journal Article | Research Support, Non-U.S. Gov't

Cell wall monoglycine cross-bridges and methicillin hypersusceptibility in a femAB null mutant of methicillin-resistant Staphylococcus aureus.

A M Strandén, K Ehlert, H Labischinski, B Berger-Bächi
A M Strandén
Institute of Medical Microbiology, University of Zürich, Switzerland.
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K Ehlert
Institute of Medical Microbiology, University of Zürich, Switzerland.
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H Labischinski
Institute of Medical Microbiology, University of Zürich, Switzerland.
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B Berger-Bächi
Institute of Medical Microbiology, University of Zürich, Switzerland.
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DOI: 10.1128/jb.179.1.9-16.1997
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ABSTRACT

The femAB operon is involved in the formation of the characteristic pentaglycine side chain of the staphylococcal peptidoglycan. Allele replacement of the femAB operon with the tetracycline resistance determinant tetK in a methicillin-resistant Staphylococcus aureus strain resulted in impaired growth, methicillin hypersusceptibility, and lysostaphin resistance. The usual pentaglycine cross-bridges were replaced by monoglycine bridges exclusively, and cross-linking of the peptidoglycan strands was drastically reduced. Complementation of the femAB null mutant by either femA or femAB resulted in the extension of the cross-bridges to a triglycine or a pentaglycine, respectively. This finding suggests that FemA is responsible for the formation of glycines 2 and 3, and FemB is responsible for formation of glycines 4 and 5, of the pentaglycine side chain of the peptidoglycan precursor. Moreover, it can be deduced that addition of the first glycine must occur by a femAB-independent mechanism.

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Cell wall monoglycine cross-bridges and methicillin hypersusceptibility in a femAB null mutant of methicillin-resistant Staphylococcus aureus.
A M Strandén, K Ehlert, H Labischinski, B Berger-Bächi
Journal of Bacteriology Jan 1997, 179 (1) 9-16; DOI: 10.1128/jb.179.1.9-16.1997

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Cell wall monoglycine cross-bridges and methicillin hypersusceptibility in a femAB null mutant of methicillin-resistant Staphylococcus aureus.
A M Strandén, K Ehlert, H Labischinski, B Berger-Bächi
Journal of Bacteriology Jan 1997, 179 (1) 9-16; DOI: 10.1128/jb.179.1.9-16.1997
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