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PHYSIOLOGY AND METABOLISM

Role of Alternative ς Factor AlgU in Encystment of Azotobacter vinelandii

Soledad Moreno, Rebeca Nájera, Josefina Guzmán, Gloria Soberón-Chávez, Guadalupe Espín
Soledad Moreno
Departamento de Microbiologı́a Molecular, Instituto de Biotecnologı́a, Universidad Nacional Autónoma de México, Cuernavaca, Morelos 62250, México
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Rebeca Nájera
Departamento de Microbiologı́a Molecular, Instituto de Biotecnologı́a, Universidad Nacional Autónoma de México, Cuernavaca, Morelos 62250, México
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Josefina Guzmán
Departamento de Microbiologı́a Molecular, Instituto de Biotecnologı́a, Universidad Nacional Autónoma de México, Cuernavaca, Morelos 62250, México
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Gloria Soberón-Chávez
Departamento de Microbiologı́a Molecular, Instituto de Biotecnologı́a, Universidad Nacional Autónoma de México, Cuernavaca, Morelos 62250, México
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Guadalupe Espín
Departamento de Microbiologı́a Molecular, Instituto de Biotecnologı́a, Universidad Nacional Autónoma de México, Cuernavaca, Morelos 62250, México
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DOI: 10.1128/JB.180.10.2766-2769.1998
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ABSTRACT

Alginate is essential for encystment in Azotobacter vinelandii. Transcription of the algD gene, which codes for GDP-mannose dehydrogenase, a key enzyme in the alginate biosynthetic pathway, is initiated at two promoters, one of which, p2, has ςE consensus sequences. AlgU is the A. vinelandii alternative ςE factor. In this study, we constructed an algU mutant (SMU88) which, as expected, is impaired in alginate production, encystment, and transcription of thealgD gene from the p2 promoter. Plasmid pJMSAT1, carrying the A. vinelandii algU gene, restored alginate production and encystment to SMU88 and to strain UW136, a naturally occurringalgU mutant. Plasmid pSMU865, carrying the A. vinelandii mucABCD genes coding for negative regulators of AlgU activity and previously shown to diminish alginate production in the wild-type strain, ATCC 9046, was shown here to impair encystment and transcription of the algD gene from the p2algU-dependent promoter. Since nonencysting strain ATCC 9046/pSMU865 produced more alginate than some encysting strains, such as UW136/pJMSAT1, we propose an AlgU role in encystment, independent of the structural role that alginate plays in mature cysts.

  • Copyright © 1998 American Society for Microbiology
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Role of Alternative ς Factor AlgU in Encystment of Azotobacter vinelandii
Soledad Moreno, Rebeca Nájera, Josefina Guzmán, Gloria Soberón-Chávez, Guadalupe Espín
Journal of Bacteriology May 1998, 180 (10) 2766-2769; DOI: 10.1128/JB.180.10.2766-2769.1998

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Role of Alternative ς Factor AlgU in Encystment of Azotobacter vinelandii
Soledad Moreno, Rebeca Nájera, Josefina Guzmán, Gloria Soberón-Chávez, Guadalupe Espín
Journal of Bacteriology May 1998, 180 (10) 2766-2769; DOI: 10.1128/JB.180.10.2766-2769.1998
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KEYWORDS

Azotobacter vinelandii
Bacterial Proteins
sigma factor

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