Article Figures & Data
Figures
- Fig. 1.
Growth of E. hirae ATCC 9790 in a defined medium. (A) Effect of KCl on growth rates in K+-deficient medium. The medium was prepared by replacing K2HPO4 in TrisM medium with phosphoric acid. Symbols: •, without NaCl; ○, with 100 mM NaCl. (B) Effect of CCCP on cell growth in TrisM medium. CCCP (50 μM), KCl (200 mM), or NaCl (200 mM) was added to the cell culture at an OD540 of 0.06 (indicated by the arrow), and cell growth was monitored by the OD540. Symbols: ○, control; ▵, TrisM plus CCCP; •, TrisM plus CCCP and KCl; ▴, TrisM plus CCCP and NaCl.
- Fig. 2.
Growth of E. hirae AS25 in TrisM medium. (A) Effect of KCl or NaCl on growth. KCl (200 mM) or NaCl (200 mM) was added to the cell culture at an OD540 of 0.05, and cell growth was monitored. Symbols: ○, control; •, TrisM plus KCl; ▴, TrisM plus NaCl. (B) Effect of NaCl on growth rates (•) and the internal levels of K+ (○) and Na+ (▵) of AS25. Cells were harvested at middle logarithmic phase and washed, and the levels of K+ and Na+ were determined with an atomic absorption spectrophotometer.
- Fig. 3.
Effect of CCCP on the growth of strains JEM2 (A) and Nak1 (B) in TrisM medium. CCCP (50 μM), KCl (200 mM), or NaCl (200 mM) was added to the cell culture at an OD540 of 0.1 (indicated by the arrows). Symbols: ○, control; ▵, TrisM plus CCCP; •, TrisM plus CCCP and KCl; ▴, TrisM plus CCCP and NaCl.
