DOI: 10.1128/JB.180.8.2244-2247.1998
Article Figures & Data
Figures
- Fig. 1.
Immunodetection of DspE in a Western blot of culture supernatant and cell fractions. Cultures of the Erwinia amylovora Hrp pathway mutant strain Ea273-K178 (lanes 1), the wild-type strain Ea273 (lanes 2), and a partial dspEdeletion mutant strain, Ea273dspEΔ1521 (lanes 3), were grown under hrp gene-inducing conditions. Molecular weight markers (BenchMark prestained protein ladder; Life Technologies, Gaithersburg, Md.) are indicated. Migration of proteins from the culture supernatant was slightly retarded due to the presence of the extracellular polysaccharide of Erwinia amylovora.
Tables
- Table 1.
Bacterial strains and plasmids used
Strain or plasmid Description Source or reference Strains E. coliDH5α F−φ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17(rK−rM+) deoR thi-1 supE44 λ−gyrA96 relA1 Life Technologies, Inc. (Gaithersburg, Md.) Erwinia amylovora Ea273 Wild type S. V. Beer Ea273-K178 Hrp secretion mutant; Tn10 mini-Km insertion in hrpJoperon Z.-M. Wei (34) Ea273dspEΔ1521 Nonpolar dspE mutant; 1,521-bp in-frame deletion in 3′ half of dspE 4 Plasmids pCPP50 pINIII113-A2-based expression vector; Apr 3 pCPP1244 3.1-kbHpaI-BamHI clone containing 5′ half ofdspE in pCPP50 4 pCPP1259 7.7-kbHpaI-HindIII clone containing dspEand dspF in pCPP50 4
