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PHYSIOLOGY AND METABOLISM

The Genes rubA and rubB for Alkane Degradation in Acinetobacter sp. Strain ADP1 Are in an Operon with estB, Encoding an Esterase, andoxyR

Walter Geißdörfer, Ruben G. Kok, Andreas Ratajczak, Klaas J. Hellingwerf, Wolfgang Hillen
Walter Geißdörfer
Lehrstuhl für Mikrobiologie, Institut für Mikrobiologie, Biochemie und Genetik der Friedrich-Alexander-Universität Erlangen-Nürnberg, 91058 Erlangen, Germany, and
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Ruben G. Kok
Laboratory for Microbiology, E. C. Slater Institute, BioCentrum Amsterdam, 1018 WS Amsterdam, The Netherlands
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Andreas Ratajczak
Lehrstuhl für Mikrobiologie, Institut für Mikrobiologie, Biochemie und Genetik der Friedrich-Alexander-Universität Erlangen-Nürnberg, 91058 Erlangen, Germany, and
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Klaas J. Hellingwerf
Laboratory for Microbiology, E. C. Slater Institute, BioCentrum Amsterdam, 1018 WS Amsterdam, The Netherlands
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Wolfgang Hillen
Lehrstuhl für Mikrobiologie, Institut für Mikrobiologie, Biochemie und Genetik der Friedrich-Alexander-Universität Erlangen-Nürnberg, 91058 Erlangen, Germany, and
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DOI: 10.1128/JB.181.14.4292-4298.1999
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ABSTRACT

Alkanes are oxidized in Acinetobacter sp. strain ADP1 by a three-component alkane monooxygenase, composed of alkane hydroxylase, rubredoxin, and rubredoxin reductase. rubA andrubB encode rubredoxin and a NAD(P)H-dependent rubredoxin reductase. We demonstrate here that single base pair substitutions inrubA or rubB lead to defects in alkane degradation, showing that both genes are essential for alkane utilization. Differences in the degradation capacity for hexadecane and dodecane in these mutants are discussed. Two genes, estBand oxyR, are located downstream of rubB, but are not necessary for alkane degradation. estB encodes a functional esterase. oxyR encodes a LysR-type transcriptional regulator, conferring resistance to hydrogen peroxide.rubA, rubB, estB, andoxyR constitute an operon, which is constitutively transcribed from a ς70 promoter, and anestB-oxyR containing message is also transcribed from an internal promoter.

  • Copyright © 1999 American Society for Microbiology
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The Genes rubA and rubB for Alkane Degradation in Acinetobacter sp. Strain ADP1 Are in an Operon with estB, Encoding an Esterase, andoxyR
Walter Geißdörfer, Ruben G. Kok, Andreas Ratajczak, Klaas J. Hellingwerf, Wolfgang Hillen
Journal of Bacteriology Jul 1999, 181 (14) 4292-4298; DOI: 10.1128/JB.181.14.4292-4298.1999

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The Genes rubA and rubB for Alkane Degradation in Acinetobacter sp. Strain ADP1 Are in an Operon with estB, Encoding an Esterase, andoxyR
Walter Geißdörfer, Ruben G. Kok, Andreas Ratajczak, Klaas J. Hellingwerf, Wolfgang Hillen
Journal of Bacteriology Jul 1999, 181 (14) 4292-4298; DOI: 10.1128/JB.181.14.4292-4298.1999
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KEYWORDS

Acinetobacter
Alkanes
DNA-binding proteins
NADH, NADPH Oxidoreductases
operon
Rubredoxins

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