Article Figures & Data
Figures
- Fig. 1.
The six porB alleles found in ET-5 complex meningococci, compared to the sequence of allele porB3-1. The sequences have been edited such that invariant bases have been removed, leaving only the variable sites. The positions in the sequence alignments of the variable bases are indicated by the vertical numbers at the top of the figure. Where a sequence varies from theporB3-1 sequence in a given allele this is shown by the appropriate letter. A period indicates that the sequence is identical to that of allele 3-1 at that position. Single changes, which are likely to be the result of de novo point mutational events, are shown as white text on black.
- Fig. 2.
The variable nucleotide sites of the nineporA alleles of ET-5 complex meningococci, compared with allele porA-2, are shown with the same conventions as used for porB in Fig. 1. The bases in those regions in loops I, IV, and V, encoding the variable regions of the gene, are indicated with arrowheads (<>). Variations likely to be the result of single de novo genetic events (point mutations, duplications, or deletions) are indicated as white text on a black background.
- Fig. 3.
Split graph of the relationships among members of the ET-5 complex. The split graphs were generated from concatenated sequences of the seven housekeeping loci with the porB andporA gene sequences. The smaller graph shows the detailed relationships within those isolates most closely related to the Norwegian ET-5 strain, H44/76. These isolates occupy the location marked by the arrow on the larger graph. Each of the edges in the graph has been annotated with the genetic change that it represents. Changes likely to be the result of single de novo genetic events (point mutations, deletions, or duplications) are shown as white text on a black background, and changes likely to be the result of recombination events are shown as boxed black text.
Tables
- Table 1.
N. meningitidis strains
Isolate Location, yr MLST allele ST porA allele porAVR1,VR2 porB allele abcZ adk aroE fumC gdh pdhC pgm H44/76 Norway, 1976 4 10 5 4 6 3 8 32 2 7,16 3-24 NG 080 Norway, 1981 4 10 5 4 6 3 8 32 2 7,16 3-24 NG144/82 Norway, 1982 4 10 5 4 6 3 8 32 2 7,16 3-63 BZ83 Holland, 1984 8 10 5 4 5 3 8 34 16 5c,10 3-1 E360 Bury St. Edmunds, UK, 1984 4 10 5 4 6 3 8 32 2 7,16 3-24 BZ 169 Holland, 1985 4 10 5 4 6 3 8 32 14 7b,16 3-14 EG 329 Germany, 1985 4 10 5 4 6 3 8 32 60 7a,16 3-24 NG PB24 Norway, 1985 4 10 5 4 6 3 8 32 57 7b,16g 3-24 F126 Stroud, UK, 1986 4 10 5 4 5 3 2 74 12 7,16b 3-24 G1960 Stroud, UK, 1987 4 10 5 4 5 3 2 74 12 7,16b 3-24 196/87 Norway, 1987 4 10 5 4 6 3 8 32 61 7b,16l 3-24 8680 Chile, 1987 4 10 5 4 6 3 8 32 41 7b,3 3-24 J129 Hackney, UK, 1988 8 10 5 4 6 3 8 33 4 19,15 3-3 K80-01508 Worcester, UK, 1989 8 10 5 4 6 3 8 33 16 5c,10 3-1 K89-01713 Worcester, UK, 1989 8 10 5 4 6 3 8 33 16 5c,10 3-1 K89-02151 Worcester, UK, 1989 8 10 5 4 6 3 8 33 16 5c,10 3-1 K89-02160 Worcester, UK, 1989 8 10 5 4 6 3 8 33 16 5c,10 3-1 K89-02224 Worcester, UK, 1989 8 10 5 4 6 3 8 33 16 5c,10 3-1 L90-00684 Hereford, UK, 1990 8 10 5 4 6 3 8 33 16 5c,10 3-1 L90-01144 Birmingham, UK, 1990 8 10 5 4 6 3 8 33 16 5c,10 3-1 L90-01047 Kidderminster, UK, 1990 8 10 5 4 6 3 8 33 16 5c,10 3-1 204/92 Cuba, 1992 8 10 5 4 6 3 8 33 4 19,15 3-8 - Table 2.
Changes in PorA protein variable regions with variants of P1.7,16
Allele VR combination Amino acid sequence VR1 VR2 porA-2 7,16 AQAANGG--ASGQVKVTKVTKA YYTKDTN-----NNLTLVP porA-60 7a,16 AQAANGGAGASGQVKVTKVTKA YYTKDTN-----NNLTLVP porA-14 7b,16 AQAANGG--ASGQV---KVTKA YYTKDTN-----NNLTLVP porA-57 7b,16g AQAANGG--ASGQV---KVTKA YYTKDTNTKDTNNNLTLVP porA-12 7,16b AQAANGG--ASGQVKVTKVTKA YYTKNTN-----NNLTLVP porA-61 7b,16l AQAANGG--ASGQV---KVTKA YYTKDTN-----DNLTLVP porA-41 7b,3 AQAANGG--ASGQV---KVTKA TLANGAN-----NTIIRVP
