Article Figures & Data
Figures
- Fig. 1.
Growth curves of R. sphaeroides f. sp.denitrificans wild type (□), MS523 mutant complemented with plasmid pRK415 (●), and MS523 mutant complemented with plasmid pMS538 (▴) under denitrifying conditions in the presence of 50 mM nitrate. OD660nm, optical density at 660 nm.
- Fig. 2.
Nondenaturing electrophoresis of periplasmic extracts (50 μg of protein) of R. sphaeroides f. sp.denitrificans wild type (WT) and MS523 mutant grown under phototrophic conditions in the presence (+) or absence (−) of 20 mM nitrate. Gels were stained for nitrate (A), nitrite (B), and N2O (C) reductase activities with dithionite-reduced methyl viologen as the electron donor or with Coomassie R250 for protein detection (D).
- Fig. 3.
Nondenaturing electrophoresis of periplasmic extracts (50 μg of protein) of the MS523 mutant containing pRK415 or pMS538 intrans. Cells were grown under phototrophic conditions in the presence (+) or absence (−) of 20 mM nitrate. Gels were stained for nitrate (A), nitrite (B), and N2O (C) reductase activities with dithionite-reduced methyl viologen as the electron donor.
- Fig. 4.
Nondenaturing electrophoresis of periplasmic extracts (50 μg of protein) of R. sphaeroides f. sp.denitrificans grown under phototrophic conditions. Added to 18-h grown cultures were 0, 0.1, or 1 mM NaNO2 or 20 mM KNO3 or N2O. Cell extracts were prepared 5 h after addition of the N oxides. Gels were stained for nitrate (A), nitrite (B), and N2O (C) reductase activities with dithionite-reduced methyl viologen as the electron donor.
