Article Figures & Data
Figures
- Fig. 1.
Nucleotide sequence of cspA1/cspA2 fromY. enterocolitica. cspA1 (upper protein sequence) andcspA2 (lower protein sequence) differ only in the 13th and 15th amino acids and in the third amino acid of the C terminus. Putative transcriptional start site (+1), putative promoter regions (−10, −35), and the cold-box are indicated. Sequence in boldface type shows the position of the A1 probe (YeA1-Dig). The underlined sequence indicated by upper facing arrows shows the putative transcriptional termination structure of the monocistronic mRNA. The underlined sequence indicated by lower facing arrows shows the transcriptional termination structure of the polycistronic mRNA. The sequence underlined by a wavy line in cspA1 can fold in an antiparallel direction to the same region of cspA2, forming an extensive secondary structure. EcoRV restriction sites, which cut at positions +73, +579, and +909, are indicated by italics.
- Fig. 2.
Northern blots of Y. enterocolitica cold shocked to 10°C. The signals were gained by a probe binding to the 5′ untranslated region of cspA1 (YeA1-Dig). Time after cold shock is given in minutes. The upper signal is approximately 900 bp and represents the bicistronic mRNA. The lower signal is approximately 450 bp and represents the monocistronic mRNA. CT, control.
- Fig. 3.
Northern blot of a dilution series of cold-shockedY. enterocolitica mRNA (10°C, 30 min) detected by using the A1-probe. The relative amounts of bi- and monocistronic mRNA fromcspA1/A2 were determined with the software Image Master 1D Elite (Amersham Pharmacia Biotech). The bicistronic mRNA shows a 300-fold increase; the monocistronic one shows a 30-fold increase.
- Fig. 4.
Northern blots of cold-shocked Y. enterocolitica mRNA obtained at different temperatures with the A1 probe. Each cold shock lasted for 20 min. Changes in the proportion of bicistronic and monocistronic mRNA are most obvious at 15 and 20°C.
- Fig. 5.
Two-dimensional gels from Y. enterocolitica. Molecular weights and pH values are indicated. MCSPs are indicated by downward arrows. (A) Windows of the broad-range gels showing proteins from pH 4 to 9 at 30°C and after a shock of 60 min to 10°C; (B) windows of the narrow-range gels from pH 5 to 6 of the control (30°C) and shock experiments to 10°C for 10 min and 60 min.
Tables
- Table 1.
Summary of results from N-terminal sequencing and MALDI
Spot no. N-terminal sequence Peptide massesa Corresponding peptide fragment sequencesa MCSP contained in spot 1 SNKMTGLVKWFDAGKG NDb B, A1 2 SNKMTGLVKWFNADKG 723.3 11-KWFDAGKG-16 (CspB or CspA1) A1, A2, B MSNKMTGLVKWFNADKG 780.3 11-KWFNADKG-16 (CspA2) 926.5 61-KGPSAVNVVAL-70 (CspA1) 940.5 61-KGPSAVNVIAL-70 (CspA2) 3 SNKMTGLVKWFNADKG 780.3 11-KWFNADKG-16 (CspA2) A2 940.5 61-KGPSAVNVIAL-70 (CspA2)
