Article Figures & Data
Figures
- Fig. 1.
(A) Scale diagram of the E. coli K10 capsule genes and flanking sequences. Recombinant plasmids used in this study are depicted as thin lines at the top. Genes within the K10 capsule cluster lie below regions 1, 2, and 3, whereas genes flanking the cluster lie below the thick lines. Arrows show direction of transcription. A scale is shown at the bottom. JS, JUMPstart sequence. (B) Diagram (not to scale) comparing the general organization of the capsule gene clusters of E. coli K5, H. influenzae, and N. meningitidis. E. coli kps homologues to H. influenzae and N. meningitidis are shown in parentheses under the corresponding genes.
- Fig. 2.
DNA sequence 5′ to region 1 of the E. coli K10 capsule gene cluster. The sequence homologous to IS3 and the JUMPstart sequence are indicated by shaded boxes. The K5 sequence is displayed under the K10 sequence where the two are homologous (boldface). The numbering of the K10 DNA starts at the beginning of the known sequence within the yghD gene. The K5 sequence is numbered from the stop codon of the yghDgene. The IS3 element interrupts regions of homology between K5 and K10. The cryptic kpsM gene is indicated by unshaded boxes. The remnant of IS110 is marked by a solid line. Δ indicates the region of K10 sequence deleted (531 bp) relative to K5. An arrow marks the transcriptional start site in the K5 sequence.
Tables
- Table 1.
Degree of amino acid homology between the putative proteins of the E. coli K10 capsule cluster and their homologues in E. coli K5, H. influenzae, and N. meningitidis
E. coli K10 protein Functiona Homologue and homology (% identity) E. coli K5 H. influenzae N. meningitidis KpsD Periplasmic protein KpsD, 34.6 KpsM ABC-transporter (membrane component) KpsM, 39.7 BexB, 28.2 CtrC, 27.5 KpsT ABC transporter (ATP-binding component) KpsT, 45.7 BexA, 46.5 CtrD, 45.8 KpsE Polysaccharide export protein KpsE, 30.0 KpsC Polysaccharide modification protein KpsC, 34.3 LipA, 39.9b KpsS Polysaccharide modification protein KpsS, 87.2 LipB, 38.5
