Article Figures & Data
Figures
- Fig. 1.
Ethanol production by permeabilized (open symbols) and intact (solid symbols) cells of the parent strain YHK142 (triangles) and the disruptant strain YHK1144 (circles) at 10°C. Cells were suspended in a buffer containing 5 mM MgCl2, 2 mM ATP, 1 mM NAD+, 30 mM Pi, 1 mM arsenate, and 150 mM MES (pH 6.9). Ethanol fermentation was initiated by addition of glucose (final concentration, 2%).
- Fig. 2.
Eadie-Hofstee plots of glucose transport in wild-type strain YHK142 (○) and CSF1 disruptant strain YHK1144 (●) at 10°C (a) and 30°C (b). The glucose concentration ranged from 0.1 to 100 mM (specific radioactivity, 6 to 740 kBq/μmol). The assay mixture was incubated for 5 s, the period during which uptake was in the linear range. Dashed lines represent a substrate concentration of 10 mM. Data were obtained from duplicate experiments.
- Fig. 3.
Arrenius plots of glucose (a) and l-leucine (b) transport at various temperatures in wild-type strain YHK142 (○) and CSF1 deletion mutant YHK1144 (●). Glucose transport was measured for 5 s with 20 mM glucose. l-Leucine transport was measured for 1 min with 1 mM l-leucine. The experiment was repeated twice.
