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ENZYMES AND PROTEINS

Cloning and Characterization of 1-Deoxy-d-Xylulose 5-Phosphate Synthase fromStreptomyces sp. Strain CL190, Which Uses both the Mevalonate and Nonmevalonate Pathways for Isopentenyl Diphosphate Biosynthesis

Tomohisa Kuzuyama, Motoki Takagi, Shunji Takahashi, Haruo Seto
Tomohisa Kuzuyama
Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan
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Motoki Takagi
Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan
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Shunji Takahashi
Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan
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Haruo Seto
Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan
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DOI: 10.1128/JB.182.4.891-897.2000
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ABSTRACT

In addition to the ubiquitous mevalonate pathway,Streptomyces sp. strain CL190 utilizes the nonmevalonate pathway for isopentenyl diphosphate biosynthesis. The initial step of this nonmevalonate pathway is the formation of 1-deoxy-d-xylulose 5-phosphate (DXP) by condensation of pyruvate and glyceraldehyde 3-phosphate catalyzed by DXP synthase. The corresponding gene, dxs, was cloned from CL190 by using PCR with two oligonucleotide primers synthesized on the basis of two highly conserved regions among dxs homologs from six genera. Thedxs gene of CL190 encodes 631 amino acid residues with a predicted molecular mass of 68 kDa. The recombinant enzyme overexpressed in Escherichia coli was purified as a soluble protein and characterized. The molecular mass of the enzyme was estimated to be 70 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 130 kDa by gel filtration chromatography, suggesting that the enzyme is most likely to be a dimer. The enzyme showed a pH optimum of 9.0, with a Vmax of 370 U per mg of protein and Kms of 65 μM for pyruvate and 120 μM for d-glyceraldehyde 3-phosphate. The purified enzyme catalyzed the formation of 1-deoxyxylulose by condensation of pyruvate and glyceraldehyde as well, with aKm value of 35 mM ford-glyceraldehyde. To compare the enzymatic properties of CL190 and E. coli DXP synthases, the latter enzyme was also overexpressed and purified. Although these two enzymes had different origins, they showed the same enzymatic properties.

  • Copyright © 2000 American Society for Microbiology
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Cloning and Characterization of 1-Deoxy-d-Xylulose 5-Phosphate Synthase fromStreptomyces sp. Strain CL190, Which Uses both the Mevalonate and Nonmevalonate Pathways for Isopentenyl Diphosphate Biosynthesis
Tomohisa Kuzuyama, Motoki Takagi, Shunji Takahashi, Haruo Seto
Journal of Bacteriology Feb 2000, 182 (4) 891-897; DOI: 10.1128/JB.182.4.891-897.2000

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Cloning and Characterization of 1-Deoxy-d-Xylulose 5-Phosphate Synthase fromStreptomyces sp. Strain CL190, Which Uses both the Mevalonate and Nonmevalonate Pathways for Isopentenyl Diphosphate Biosynthesis
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Cloning and Characterization of 1-Deoxy-d-Xylulose 5-Phosphate Synthase fromStreptomyces sp. Strain CL190, Which Uses both the Mevalonate and Nonmevalonate Pathways for Isopentenyl Diphosphate Biosynthesis
Tomohisa Kuzuyama, Motoki Takagi, Shunji Takahashi, Haruo Seto
Journal of Bacteriology Feb 2000, 182 (4) 891-897; DOI: 10.1128/JB.182.4.891-897.2000
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KEYWORDS

Hemiterpenes
Mevalonic Acid
Organophosphorus Compounds
Streptomyces
Transferases

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