Article Figures & Data
Figures
- Fig. 1.
(A) Features of the yhaG region of theB. subtilis chromosome. Promoter regions and translation start and stop codons are underlined. The start point of transcription is marked by an asterisk and an arrow. The Shine-Dalgarno sequence (SD) is indicated by a dashed underline. A sequence corresponding to a Rho-independent transcription terminator is indicated by a double underline. Trinucleotide repeats constituting a putative TRAP binding site are shaded. Nucleotide coordinates shown are from 1074054 to 1074773 according to the B. subtilis genome database (8). (B) Predicted topological model of the YhaG protein, generated by the TopPred II computer program using a window size of 21 (5). Oval structures represent alpha-helical transmembrane spans. Predicted amino acid residues at the membrane interface are indicated.
Tables
- Table 1.
Regulation of yhaG expression and effect of multicopy expression of the yhaG leader region ontrpE and trpG gene expression
Fusion (integrated in amyElocus) Genetic background Plasmid β-Galactosidase sp act of strain grown under conditiona −Tryptophan + Tryptophan yhaG′-′lacZ mtrB+ None 20 1.1 yhaG′-′lacZ ΔmtrB None 2,270 2,200 trpE′-′lacZ mtrB+ None 13 0.1 trpE′-′lacZ mtrB+ pHY300PLKb 11 0.3 trpE′-′lacZ mtrB+ pJPS647c 248 246 trpE′-′lacZ mtrB264 None 215 187 trpG′-′lacZ mtrB+ None 2.3 0.4 trpG′-′lacZ mtrB+ pHY300PLK 1.9 0.4 trpG′-′lacZ mtrB+ pJPS647 11 9.7 trpG′-′lacZ mtrB264 None 13 12 ↵a Strains were grown overnight in Vogel-Bonner medium, subcultured in the same medium with or without 50 μg of l-tryptophan per ml, and grown for three to four generations. Assays were performed in duplicate on three separate occasions. Values are presented in Miller units.
↵b Parental multicopy shuttle vector.
↵c pHY300PLK containing a 352-bp yhaGpromoter-leader fragment.
