The GacS Sensor Kinase Regulates Alginate and Poly-β-Hydroxybutyrate Production in Azotobacter vinelandii

Identification of the locus responsible for the suppression of mucoidy.Mini-Tn5-lacZ1 (6) mutagenesis of plasmid pSMU588 was carried out to identify the locus responsible for the reduction of alginate. A pSMU588::mini-Tn5-lacZ1 derivative that no longer suppressed mucoidy was isolated and named pSMU588-21. Alginate production of ATCC 9046 carrying this plasmid was determined and the results are shown in Table 1.

We determined 3 kb of nucleotide sequence around the mini-Tn5-lacZ1 insertion. DNA sequence analysis revealed one open reading frame encoding a polypeptide of 905 amino acid residues, sharing similarity with transmembrane sensor kinases belonging to signal transduction proteins of the family of two-component regulators (12). Among those with the highest identity (65%) was GacS (previously called LemA), which is present in the followingPseudomonas species: P. syringae (13),P. viridiflava (15), P. fluorescens(5), P. aureofaciens (3), and P. tolaasii (11). In P. syringae, GacS and its cognate response regulator, GacA, are required for the production of the toxin syringomycin and for extracellular proteases (27); in P. viridiflava, GacS controls protease, pectate lyase and alginate production (15); and in P. fluorecens, GacS controls antibiotic production (5). A gacShomolog, rpfA, which regulates the production of virulence factors, has been reported in the phytopathogenic bacteriumErwinia carotovora (8). In P. aeruginosa and P. aureofaciens, GacA, the GacS cognate response regulator, has been shown to be a global activator regulating several products and virulence factors via acyl-l-homoserine lactones (3, 26).

The A. vinelandii GacS amino acid sequence contains specific motifs typical of the ArcB subfamily, histidine protein kinases that are characterized by containing, in addition to the two transmembrane domains and the orthodox transmitter H1 domain, a response regulator D1 domain and a second phosphorylatable histidine, H2 (9, 14). The exact location of the mini-Tn5-lacZ1 mutation withingacS was mapped between codons encoding amino acids 735 and 736.

Plasmid pMC15.To rule out the possibility that plasmid pSMU588-21 no longer suppressed alginate production due to a polar effect of the gacS::mini-Tn5-lacZ1insertion rather than to the lack of the gacS gene product, oligonucleotides gacS1 (5′-AAGCGGAGCTCGAGCCGTCAGG-3′) and gacS2 (5′-ACGGTGCCGTCTCGAGTTTCCGCTC-3′) were used to isolate, by PCR, a fragment containing the gacS gene flanked by the ATG codon (200 bp upstream) and the stop codon (20 bp downstream); this fragment was cloned into plasmid pKT230 (1). The resultant plasmid, pMC15, was found to have a much stronger effect on alginate production (Table 1), confirming the negative effect of gacS on alginate production and ruling out the possibility of a polar effect.

Construction and characterization of gacS mutants.Sensor kinases of the two-component regulatory systems usually act as positive regulators by phosphorylating the cognate response regulator. To investigate a positive role of gacS on alginate biosynthesis, ATCC 9046 derivatives carrying gacS mutations were constructed: in A. vinelandii the insertion of Ω cassettes into genes with the same orientation as the direction of transcription produces nonpolar mutations which allow transcription of the downstream genes in the same operon (21, 24). Plasmid pMC5, a pBluescript KS(+) plasmid which carries a 2.5-kbSmaI DNA fragment including gacS, was used to construct gacS::Ω-Sp mutations. A 2-kb fragment containing a spectinomycin resistance gene (Ω-Sp) from plasmid pHP45Ω-Sp (7) was inserted into the uniqueEcoRI site present within gacS. Plasmids pMC7 and pMC8 with the cassette inserted in both orientations were selected and introduced into strain ATCC 9046. Sp^r transformants were selected. Strains JM1 and JM2 were isolated and were shown, by Southern blot analysis, to carry the gacS::Ω-Sp nonpolar and polar mutations, respectively (data not shown). RNA isolated from strains JM1, JM2, and ATCC 9046 were hybridized with a 700-bp fragment corresponding to the 3′ end of gacS. Hybridization of RNA from the wild type with that of the gacS nonpolar JM1 mutant but not with that of the JM2 polar mutant was observed (data not shown). Both gacS mutant strains produced threefold less alginate than the parental strain, ATCC 9046, when grown on solid medium (Table 1). When cultures of these mutants were grown on liquid medium, a 30- to 60-fold reduction in alginate production was detected. These data indicate that the negative effect on alginate production observed is due to the lack of the gacS gene product and not to a polar effect.

GacS also controls PHB production.PHB granules were visible in cells of wild-type ATCC 9046 under a light microscope; however, no PHB granules were observed in cells of strains JM1 or JM2. PHB accumulation in these strains was determined. As shown in Table 1, either a gacS mutation or plasmid pMC15 caused a significant reduction in PHB accumulation. Electron microscopic examination of cultures of ATCC 9046, JM2, and ATCC 9046/pMC15 was carried out as described previously (21), and the results are shown in Fig.1. In contrast to the wild-type ATCC 9046, where big PHB granules are observed, strains JM2 and ATCC 9046/pMC15 appear to contain numerous very small PHB granules and disorganized or amorphic white structures that appear to contain PHB. These observations indicate that GacS also regulates PHB accumulation.

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Fig. 1.

Electron micrographs of A. vinelandii cells grown for 48 h in Burk's medium supplemented with 2.0% sucrose. (A) Strain ATCC 9046; (B) strain JM2; (C) strain ATCC 9046/pMC15. Arrows indicate small PHB granules. Bars, 0.4 μm.

The effect of the gacS mutation on alginate and PHB production indicates that GacS plays a positive role in the regulation of polymer synthesis in A. vinelandii. This role is likely due to the kinase activity of GacS that results in phosphorylation of GacA, leading to activation of alginate and PHB genes. On the other hand, in the presence of the gacS gene cloned into the pKT230 vector, with a copy number of 15 to 20 (4), the synthesis of these polymers is significantly reduced. A similar observation was reported for the Escherichia coli histidine protein kinase RcsC, which, together with RcsA, positively regulates synthesis of colanic acid, a component of the capsule conferring resistance to desiccation in E. coli (31). Cells carrying a multicopy rcsC plasmid were shown to reduce the level of colanic acid (10). However, the negative effects observed when two-component regulatory systems are present on multicopy plasmids do not necessarily reflect a natural situation; thus, the reduced alginate level may represent an artifact.

The algD gene is a target of signal transduction by GacS. algD, the gene encoding GDP-mannose dehydrogenase, the key enzyme in the alginate biosynthetic pathway, seems to be highly regulated, since its transcription can initiate from three different sites: p1, a ς⁷⁰ type of promoter (2); p2, controlled by ς^E (23); and p3. We determined whether the effect of GacS on alginate biosynthesis was exerted on the transcription of the algD gene by measuring the β-galactosidase activity of strain WI12, an ATCC 9046 derivative carrying an algD::lacZ gene fusion (in the presence and absence of plasmid pMC15), as well as of strain WI12-1, a WI12 derivative carrying thegacS::Ω-Sp nonpolar mutation. The β-galactosidase activity was measured as reported by Miller (22); 1 U corresonds to 1 nmol ofO-nitrophenyl-β-d-galactoside hydrolyzed per min per μg of protein. Protein was determined by the Lowry method (17). All measurements were done in triplicate. The β-galactosidase activity was measured during growth on Burk's medium supplemented with 2% sucrose (Fig. 2A). In both the WI12-1 and WI12/pMC15 strains the β-galactosidase activity was reduced (Fig. 2B). We determined which of thealgD promoters was regulated by GacS. Primer extension ofalgD on RNA isolated from strains JM1 and ATCC 9046/pMC15 was performed as previously described (2). No primer extension products were detected with RNA from these strains grown for 48 h in liquid Burk's medium supplemented with 2% sucrose (Fig.3). This result is in agreement with the low β-galactosidase activity level detected under the same conditions. These data show that, in regulating alginate synthesis GacS exerts influence on transcription of algD from its three promoters. Similarly, in Pseudomonas fluorecens GacS and GacA regulate gene expression by influencing the ς^Slevels in addition to being required for expression of genes not regulated by ς^S (33). Thus, the GacSA system controls expression from promoters recognized by different sigma factors. In this regard, it is possible that the putative ς⁷⁰ algD promoter, P1, may instead be a ς^S-dependent promoter.

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Fig. 2.

Growth (A) and β-galactosidase activity (B) on Burk's medium supplemented with 2% sucrose are shown for the strains WI12 (squares and black bars), WI12-1 (triangles and grey bars), and WI12/pMC15 (circles and white bars). Prot., protein.

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Fig. 3.

Primer extension analysis of algDtranscription. (A) DNA sequence of the 5′ end of algD. Triangles indicate the start sites of algD transcription. The ATG initiation codon is overlined. (B) Primer extension of thealgD gene in strains ATCC 9046 (lane 1), JM2 (lane 2), and ATCC 9046/pMC15 (lane 3).

GacA must mediate signal transduction between GacS and algD. Members of our group have identified in the A. vinelandiichromosome a gene homologous to gacA (M. Castañeda, J. Sánchez, and G. Espı́n, unpublished results). Whether GacA directly interacts with the algD promoter region remains to be determined. In P. aureofaciens, GacA functions upstream of the PhzR regulator, in the signal transduction pathway that regulates antibiotic synthesis via acyl-homoserine lactones (3); thus, it is possible that in A. vinelandiiother regulatory proteins mediate signal transduction between GacS, GacA, and algD.

It is difficult to speculate on the target of signal transduction by GacS in PHB synthesis, since regulation of the A. vinelandii phb biosynthetic genes is presently unknown. The A. vinelandii gacS mutation caused a PHB leaky phenotype and affected the size of the PHB granules. Similarly, in Ralstonia eutropha, phaP, a locus causing a PHB leaky phenotype, encodes a PHB-binding protein that determines the size of polyhydroxyalkanoic acid granules (34). Thus,gacS could be involved in the control of a gene homologous to phaP.

Effect of the gacS mutation on encystment.Alginate has been shown to be essential for the formation of mature cysts (2). As shown above, strain JM1 produces some alginate on plates of Burk's medium supplemented with 2% sucrose, and strains producing similar or lower levels of alginate are still able to form cysts (23); we analyzed the encysting capacity of strain JM1, measuring desiccation resistance of cultures induced for encystment as previously described (2). A reduction of more than 1,000-fold in encystment frequency was observed in thegacS mutant JM1 (Table 1). However under encysting conditions, i.e., in n-butanol plates, alginate production by strain JM1 was very low. Thus, as is the case with other mutants impaired in alginate production, strain JM1 is unable to form desiccation-resistant cysts. GacS may affect this differentiation process exclusively via its effect on alginate biosynthesis; however, whether this global regulator is required for expression of other genes involved in encystment remains to be investigated.

Nucleotide sequence accession number.The nucleotide sequence determined in this study has the GenBank accession no. AF197912.