Article Figures & Data
Figures
- Fig. 1.
Multiple sequence alignment of the GerN protein against its homologues Gba_1701 of B. anthracis, GrmA ofB. megaterium, NapA of E. hirae, locus CAA51756 of L. lactis, the N-terminal domains of KefB and KefC of E. coli, and the N-terminal domain of YjbQ ofB. subtilis. Solid boxes indicate identical amino acids, and shaded boxes indicate conserved amino acids, for 50% of the sequences.
- Fig. 2.
Effect of a gerN mutation on OD loss during germination. (A) Germination of B. cereus 569 andgerN1 mutant spore suspensions in response to 5 mM inosine plus 10 mM NaCl (circles and squares, wild-type and mutant spores, respectively) or 10 mM l-alanine plus 10 mM NaCl (diamonds and triangles, wild-type and mutant spores, respectively). (B) Germination of B. cereus and gerN1 mutant spore suspensions in response to 50 μM inosine plus 10 mM NaCl (circles and squares, wild-type and mutant spores, respectively) or 50 μM l-alanine plus 10 mM NaCl (diamonds and triangles, wild-type and mutant spores, respectively).
- Fig. 3.
Germination of spore suspensions of B. cereus 569 (squares) and the gerN mutant (triangles) in calcium DPA.
- Fig. 4.
(A) Electron micrograph of B. cereus gerN1 mutant spores after germination in 5 mM inosine and 10 mM NaCl for 90 min. (B) Electron micrograph of B. cereus gerN1 mutant spores after germination and outgrowth in NB for 210 min. Bar, 1 μm.
Tables
- Table 1.
Release of cations during the germination ofB. cereus 569 and gerN null spores in 5 mM inosine
Time (min) Cation release (mg/g of spores)a during germination of: B. cereus 569 gerN1 mutant Ca2+ Mg2+ K+ Ca2+ Mg2+ K+ 1 0.1 0.013 0.02 0.1 0.018 0.0 2 0.63 0.09 0.052 ND ND ND 10 2.74 0.379 0.0 0.12 0.01 0.029 30 3.26 0.689 0.0 0.23 0.031 0.033 90 ND ND ND 3.02 0.327 0.0 ↵a ND, not determined.
