Novel Motility Mutants ofSynechocystis Strain PCC 6803 Generated by In Vitro Transposon Mutagenesis

DOI: 10.1128/JB.183.20.6140-6143.2001

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Fig. 1.
Comparison of sll0183, sll0301, and sll0414, with black boxes indicating identical or conserved residues in all polypeptides and gray boxes indicating residues conserved in two polypeptides. The region with pentapeptide repeats is underlined; the tetratricopeptide motif of sll0183 is indicated by black circles.

Table 1.

Mutants generated by transposon mutagenesis

Phenotype	Position(s) of transposon insertion(s)	Size of gene, amino acids (nucleotides)	Gene designation	Putative gene function and/or protein domain
Nonmover	−13, 776	369 (1,107)	slr0161	PilT1
Nonmover	521	405 (1,215)	slr0162	PilC
Nonmover	99, 448, 534, 1107	692 (2,076)	sll0058	Chaperone
Nonmover	617	354 (1,062)	sll0415	ABC transporter
Nonmover	276, 606, 664	324 (972)	sll0564	Ubiquinone methyltransferase-like
Nonmover	270	133 (399)	sll0565	E/Q-rich protein downstream of ubiquinone methyltransferase
Nonmover	911	416 (1,248)	sll1575	Eukaryotic-type serine/threonine protein kinase
Nonmover	953	337 (1,011)	slr1991	Adenylyl cyclase (cya1)
Nonmover	89	158 (474)	slr2016	Part of a gene locus (slr2015-2019) which includes an ABC transporter (slr2019) and pilA-like gene (slr2016)
Nonmover	83, 244, 583, 859	360 (1,080)	slr2017	Part of a gene locus (slr2015-2019)
Nonmover	78, 2280	799 (2,397)	slr2018	Part of a gene locus (slr2015-2019)
Slowmover	437	374 (1,122)	slr0358	E/P-rich
Nonmover	−3, 443, 1057	535 (1,605)	slr1301	E/Q-rich coiled coil
Nonmover	147	134 (402)	slr1964	Q-rich
Nonmover	64, 348, 502	259 (777)	sll0183	Pentapeptide repeat domain and tetratricopeptide domain
Slowmover	342	286 (858)	sll0414	Pentapeptide repeat domain
Nonmover	97	169 (507)	sll0301	Pentapeptide repeat domain

Table 2.

Novel mutants with high E, Q, and P percentages or repeated motifs

Gene	Size		Insertion position(s)	Amino acid composition (% E/% Q/% P)^a	Signal peptide	Coils	Transmembrane helices	PFAM designation(s)
Gene	Residues	Nucleotides	Insertion position(s)	Amino acid composition (% E/% Q/% P)^a	Signal peptide	Coils	Transmembrane helices	PFAM designation(s)
slr0358	374	1,122	437	13/4.3/10.4	No	No	No	No hits
sll0565	133	399	270	10.5/11.3/0.8	No	Yes	Yes	No hits
slr1964	134	402	147	5.2/10.4/0.75	No	Yes	Yes	No hits
slr1301	535	1,605	3, 443, 1057	13.6/13.6/1.9	No	Yes	No	No hits
sll0183	259	777	64, 348, 502	5/4.6/3.1	Yes (18/19)^b	No	Yes	00515, 00805
sll0414	286	858	342	4.5/9.1/3.5	No	No	No	00805
sll0301	169	507	97	3/2.4/1.2	Yes (32/33)	No	Yes	00805

↵a Numbers in bold represent amino acid percentages higher than 10% (usual levels are 3 to 5%).
↵b Numbers in parentheses represent putative signal peptide cleavage sites.