Fig. 1.

Topological diagram of Tet(L) highlighting the region deleted in construction of Tet(L)-12. The gray bars show the positions of motifs that were noted by Paulsen et al. (21) in 12- and/or 14-TMS drug/H⁺ antiporters. The twoMslI sites used in construction of Tet(L)-12 are indicated by the scissors arrowheads.