DOI: 10.1128/JB.184.12.3392-3395.2002
ABSTRACT
It was determined that the dTDP-rhamnose synthesis gene, rmlD, could be inactivated in Mycobacterium smegmatis only in the presence of a rescue plasmid carrying functional rmlD. Hence, dTDP-rhamnose biosynthesis is essential for the growth of mycobacteria and the targeting of dTDP-rhamnose synthesis for new tuberculosis drugs is supported.
- Copyright © 2002 American Society for Microbiology
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- Article
- ABSTRACT
- Constructing the rmlD replacement plasmid (pFP201) and obtaining the first homologous recombination event.
- Construction of an rmlD rescue plasmid.
- Second-crossover attempts and events.
- M. smegmatis YM202 will not grow at 42°C.
- The wbbL gene is transcribed and translated in YM202.
- Application of results to M. tuberculosis.
- ACKNOWLEDGMENTS
- FOOTNOTES
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