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PHYSIOLOGY AND METABOLISM

The metDd-Methionine Transporter Locus of Escherichia coli Is an ABC Transporter Gene Cluster

József Gál, Attila Szvetnik, Róbert Schnell, Miklós Kálmán
József Gál
Institute for Biotechnology, Bay Zoltán Foundation for Applied Research, and Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, H-6701 Szeged, Hungary
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  • For correspondence: galj2@mail.nih.gov
Attila Szvetnik
Institute for Biotechnology, Bay Zoltán Foundation for Applied Research, and Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, H-6701 Szeged, Hungary
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Róbert Schnell
Institute for Biotechnology, Bay Zoltán Foundation for Applied Research, and Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, H-6701 Szeged, Hungary
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Miklós Kálmán
Institute for Biotechnology, Bay Zoltán Foundation for Applied Research, and Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, H-6701 Szeged, Hungary
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DOI: 10.1128/JB.184.17.4930-4932.2002
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    FIG. 1.

    Structure of the predicted promoter upstream of abc. The −35 and −10 boxes are indicated in boldface type, and the tandem 100% and 62.5% MET boxes are shown as boxed sequences. The predicted transcriptional start nucleotide is underlined; stars indicate homology.

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  • TABLE 1.

    β-Galactosidase specific activities in the JM109 host strain in liquid M9 glucose minimal cultures

    PlasmidsSp act (Miller units) with:
    No added l-methionine100 μg of l-methionine/ml
    pRS415, pBAD3319.319.0
    pROMET1, pBAD3326,6438,476
    pROMET1, pMJ3313,3322,145
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The metDd-Methionine Transporter Locus of Escherichia coli Is an ABC Transporter Gene Cluster
József Gál, Attila Szvetnik, Róbert Schnell, Miklós Kálmán
Journal of Bacteriology Sep 2002, 184 (17) 4930-4932; DOI: 10.1128/JB.184.17.4930-4932.2002

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The metDd-Methionine Transporter Locus of Escherichia coli Is an ABC Transporter Gene Cluster
József Gál, Attila Szvetnik, Róbert Schnell, Miklós Kálmán
Journal of Bacteriology Sep 2002, 184 (17) 4930-4932; DOI: 10.1128/JB.184.17.4930-4932.2002
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  • Article
    • ABSTRACT
    • Uptake of d-methionine.
    • Uptake of α-methyl methionine.
    • Uptake of l-methionine.
    • Regulation of transcription of the metD locus.
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
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KEYWORDS

ATP-binding cassette transporters
Amino Acid Transport Systems
Escherichia coli
methionine
Multigene Family

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