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GENETICS AND MOLECULAR BIOLOGY

vanE Gene Cluster of Vancomycin-Resistant Enterococcus faecalis BM4405

Lorena Abadía Patiño, Patrice Courvalin, Bruno Perichon
Lorena Abadía Patiño
Unité des Agents Antibactériens, Institut Pasteur, 75724 Paris Cedex 15, France
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Patrice Courvalin
Unité des Agents Antibactériens, Institut Pasteur, 75724 Paris Cedex 15, France
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Bruno Perichon
Unité des Agents Antibactériens, Institut Pasteur, 75724 Paris Cedex 15, France
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  • For correspondence: brunoper@pasteur.fr
DOI: 10.1128/JB.184.23.6457-6464.2002
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Article Information

DOI 
https://doi.org/10.1128/JB.184.23.6457-6464.2002
PubMed 
12426332

Published By 
American Society for Microbiology Journals
History 
  • Published online December 1, 2002.

Copyright & Usage 
Copyright © 2002 American Society for Microbiology

Author Information

  1. Lorena Abadía Patiño,
  2. Patrice Courvalin and
  3. Bruno Perichon*
  1. Unité des Agents Antibactériens, Institut Pasteur, 75724 Paris Cedex 15, France
  • ↵* Corresponding author. Mailing address: Unité des Agents Anti-bactériens, Institut Pasteur, 25, Rue du Dr. Roux, 75724 Paris Cedex 15, France. Phone: 33 1 45 68 83 18. Fax: 33 1 45 68 83 19. E-mail: brunoper{at}pasteur.fr .

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vanE Gene Cluster of Vancomycin-Resistant Enterococcus faecalis BM4405
Lorena Abadía Patiño, Patrice Courvalin, Bruno Perichon
Journal of Bacteriology Dec 2002, 184 (23) 6457-6464; DOI: 10.1128/JB.184.23.6457-6464.2002

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vanE Gene Cluster of Vancomycin-Resistant Enterococcus faecalis BM4405
Lorena Abadía Patiño, Patrice Courvalin, Bruno Perichon
Journal of Bacteriology Dec 2002, 184 (23) 6457-6464; DOI: 10.1128/JB.184.23.6457-6464.2002
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  • Top
  • Article
    • Recombinant DNA techniques.
    • Plasmid construction.
    • (i) Plasmid pAT664.
    • (ii) Plasmids pAT667 and pAT668.
    • Probes and hybridization.
    • PCR and nucleotide sequencing.
    • Computer analysis of sequence data.
    • Analysis of peptidoglycan precursors.
    • Pulsed-field gel electrophoresis.
    • RNA techniques. (i) Extraction of total RNA.
    • (ii) Northern analysis.
    • (iii) RT-PCR experiments.
    • (iv) Primer extension analysis.
    • RESULTS AND DISCUSSION
    • ACKNOWLEDGMENTS
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

KEYWORDS

Bacterial Proteins
Carboxypeptidases
Drug Resistance, Microbial
Endopeptidases
Enterococcus faecalis
Genes, Bacterial
Ligases
membrane proteins
Racemases and Epimerases
Serine-Type D-Ala-D-Ala Carboxypeptidase
Trans-Activators
vancomycin resistance

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