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SIGNAL TRANSDUCTION

Identification and Molecular Characterization of the Mg2+ Stimulon of Escherichia coli

Shu Minagawa, Hiroshi Ogasawara, Akinori Kato, Kaneyoshi Yamamoto, Yoko Eguchi, Taku Oshima, Hirotada Mori, Akira Ishihama, Ryutaro Utsumi
Shu Minagawa
1Department of Bioscience and Biotechnology, Graduate School of Agriculture, Kinki University, 3327-204 Nakamachi, Nara 631-8505
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Hiroshi Ogasawara
1Department of Bioscience and Biotechnology, Graduate School of Agriculture, Kinki University, 3327-204 Nakamachi, Nara 631-8505
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Akinori Kato
1Department of Bioscience and Biotechnology, Graduate School of Agriculture, Kinki University, 3327-204 Nakamachi, Nara 631-8505
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Kaneyoshi Yamamoto
1Department of Bioscience and Biotechnology, Graduate School of Agriculture, Kinki University, 3327-204 Nakamachi, Nara 631-8505
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Yoko Eguchi
1Department of Bioscience and Biotechnology, Graduate School of Agriculture, Kinki University, 3327-204 Nakamachi, Nara 631-8505
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Taku Oshima
2Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Ikoma 630-0101
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Hirotada Mori
2Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Ikoma 630-0101
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Akira Ishihama
3Division of Molecular Biology, Nippon Institute for Biological Science, Ome, Tokyo 198-0024, Japan
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Ryutaro Utsumi
1Department of Bioscience and Biotechnology, Graduate School of Agriculture, Kinki University, 3327-204 Nakamachi, Nara 631-8505
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  • For correspondence: utsumi@nara.kindai.ac.jp
DOI: 10.1128/JB.185.13.3696-3702.2003
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ABSTRACT

Transcription profile microarray analysis in Escherichia coli was performed to identify the member genes of the Mg2+ stimulon that respond to the availability of external Mg2+ in a PhoP/PhoQ two-component system-dependent manner. The mRNA levels of W3110 in the presence of 30 mM MgCl2, WP3022 (phoP defective), and WQ3007 (phoQ defective) were compared with those of W3110 in the absence of MgCl2. The expression ratios of a total of 232 genes were <0.75 in all three strains (the supplemental data are shown at http://www.nara.kindai.ac.jp/nogei/seiken/array.html ), suggesting that the PhoP/PhoQ system is involved directly or indirectly in the transcription of these genes. Of those, 26 contained the PhoP box-like sequences with the direct repeats of (T/G)GTTTA within 500 bp upstream of the initiation codon. Furthermore, S1 nuclease assays of 26 promoters were performed to verify six new Mg2+ stimulon genes, hemL, nagA, rstAB, slyB, vboR, and yrbL, in addition to the phoPQ, mgrB, and mgtA genes reported previously. In gel shift and DNase I footprinting assays, all of these genes were found to be regulated directly by PhoP. Thus, we concluded that the phoPQ, mgrB, mgtA, hemL, nagA, rstAB, slyB, vboR, and yrbL genes make up the Mg2+ stimulon in E. coli.

  • Copyright © 2003 American Society for Microbiology
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Identification and Molecular Characterization of the Mg2+ Stimulon of Escherichia coli
Shu Minagawa, Hiroshi Ogasawara, Akinori Kato, Kaneyoshi Yamamoto, Yoko Eguchi, Taku Oshima, Hirotada Mori, Akira Ishihama, Ryutaro Utsumi
Journal of Bacteriology Jul 2003, 185 (13) 3696-3702; DOI: 10.1128/JB.185.13.3696-3702.2003

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Identification and Molecular Characterization of the Mg2+ Stimulon of Escherichia coli
Shu Minagawa, Hiroshi Ogasawara, Akinori Kato, Kaneyoshi Yamamoto, Yoko Eguchi, Taku Oshima, Hirotada Mori, Akira Ishihama, Ryutaro Utsumi
Journal of Bacteriology Jul 2003, 185 (13) 3696-3702; DOI: 10.1128/JB.185.13.3696-3702.2003
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KEYWORDS

Escherichia coli
Escherichia coli Proteins
Gene Expression Regulation, Bacterial
magnesium
Oligonucleotide Array Sequence Analysis
signal transduction

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