Article Figures & Data
Figures
- FIG. 1.
Organization of C. thermocellum scaffoldin-related genes. cipA and the three genes olpB, orf2, and olpA are located in tandem within a gene cluster. sdbA is located on a distinct region of the chromosome. The proteins OlpB, Orf2p, and SdbA serve as anchoring proteins that connect the CipA scaffoldin to the cell surface. In contrast, OlpA selectively binds individual cellulases to the cell surface. The numbers above the gene in the scheme refer to its size (in base pairs).
- FIG. 2.
Transcript levels of the cipA gene and the genes coding for the cipA-specific anchoring proteins, olpB, orf2, and sdbA. RPAs were performed with the relevant 32P-labeled antisense probe as described earlier (11), using RNA from exponential (A) or stationary (B) phase cultures, grown on either cellobiose or crystalline cellulose. Different amounts (in micrograms) of RNA hybridized with the probes are indicated at the top of each lane. Autoradiographs of the respective RPA products were visualized using a phosphorimager system. Negative control lanes (NC) contained RNA from yeast. Lane M corresponds to markers (in base pairs). The full-length probe is shown in lane P. Values at right indicate the sizes of the undigested full-length antisense probes and the estimated sizes of the protected products in bases (b).
- FIG. 3.
mRNA expression of cipA and scaffoldin-related genes as determined by Northern blot analysis. Northern blot analysis was performed using total RNA isolated from cultures grown on either cellobiose (lanes 1 and 2) or crystalline cellulose (lanes 3 and 4), during exponential (lanes 1 and 3) and stationary (lanes 2 and 4) phases of growth. The isolated RNA (15 μg) was loaded and separated on agarose gels and then transferred to nitrocellulose membranes. The blot was hybridized subsequently with cipA, olpB, sdbA, and orf2 probes. The sizes of the16S and 23S rRNA and the estimated sizes of the transcripts are indicated in bases (b). Arrows denote larger-than-expected sized transcripts labeled by the designated probe (see text for details).
- FIG. 4.
Transcript levels of cipA and scaffoldin-related genes as a function of growth rate. The amounts of the respective mRNA were determined by RPA and converted to the number of transcripts per cell based on the average measured amount of total RNA in a single cell (0.18 pg/cell). Values represent the average of several (at least 3) measurements at an accuracy of ± 25%. Continuous cultures were operated under carbon (cellobiose) limitation at dilution rates between 0.04 ≤ μ ≤ 0.21; batch cultures were grown to exponential phase on either cellulose or cellobiose at rates of μ = 0.23 and 0.35 h−1, respectively.
- FIG. 5.
Mapping of the 5′ terminus of cipA by primer extension analysis. (A) 32P-labeled oligonucleotide was hybridized to mRNA obtained from C. thermocellum grown under the following conditions: continuous culture limited on cellobiose, diluted to a rate of 0.1 h−1 (lane 1); exponential-phase culture grown on Avicel (lane 2); and exponential-phase culture grown on cellobiose (lane 3). Dideoxynucleotide sequence reactions were performed using the same primers employed in the reverse transcriptase reactions. The positions of the transcriptional start points are indicated by asterisks on the inferred non-template-strand sequences. The products of primer extension are indicated by P1 and P2. (B) Sequence data for the regulatory region of cipA. The respective transcriptional start points (P1 and P2) are indicated. The consensus B. subtilis σA and σL promoter sequences are framed at the homologous sites of the C. thermocellum sequence. The proposed Shine-Dalgarno (SD) site and the initiating ATG codon are indicated.
Tables
- TABLE 1.
Transcript levels of the scaffoldin gene, cipA, and the genes encoding for anchoring proteins olpB, orf2p, and sdbA in C. thermocellum cultures.
Gene No. of transcripts/cella Cellobiose Crystalline cellulose Exponential Late exponential Exponential Late exponential cipA 9 8 23 7 olpB 2 4 11 3 orf2p 13 14 19 11 sdbA 3 4 4 6 ↵a Values represent the average ± 15% standard error of at least three determinations of the mRNA level.
- TABLE 2.
Transcript levels of designated genes during continuous culture under nitrogen limitation
Gene No. of transcripts per cella at dilution rate (h−1) 0.14 0.11 0.07 cipA 8 14 28 olpB 9 17 31 orf2p 10 14 25 sdbA 3.9 3.5 5 ↵a Values represent the average ± 15% standard error of at least three determinations of the mRNA level.
- TABLE 3.
Proposed promoter sequences in the regions upstream of selected cellulosome-related genes
Gene Proposed promoter sequencea cipA TAA-CACT—N16—TTGCTTGGTTTGT olpB TAAGCACT—N15—GTGATTACGACGA orf2p TAA CATA—N14—TTGCTGTTTTAAAGA celS TAA-CACA—N15—CAGCTTAGTTTTATG Consensus TAA-CACW—Nn—NTGCTT Consensus σL TGG-CACN—N5—TTGCAb
