Article Figures & Data
Figures
- FIG. 1.
Alignment of TPS of T. thermophilus RQ-1. The deduced amino acid sequence of otsA of RQ-1 was aligned with those of homologous proteins: Styph, Salmonella enterica serovar Typhimurium (GenPept AF213176); Rhisp., Rhizobium sp. (GenPept AAB91813); Mtube, M. tuberculosis (GenPept CAB08713); Rsola, Ralstonia solanacearum (GenPept CAD17882); Ecoli, E. coli (GenPept AAC74966). Black shading represents identity, and gray shading represents conservative changes.
- FIG. 2.
Verification of the internal deletion in otsA and otsB. Southern blots of genomic DNA from T. thermophilus RQ-1 and two kanamycin-resistant clones (RQ-1M6 and RQ-1M8) are shown. Bands were identified by hybridization with a 2.05-kbp fragment corresponding to otsA and otsB (A) or kat (B). Lanes 1, 4, and 7, RQ-1M6; lanes 2, 5, and 8, RQ-1M8; lanes 3, 6, and 9, RQ-1. (C) Genetic organization of trehalose-synthesizing genes in the chromosomes of the wild type and an RQ-1 deletion mutant.
- FIG. 3.
Growth of wild-type and mutant T. thermophilus strains in TD medium with 0% (○), 3% (•), 4% (▵), 5% (▴), and 6% (▪) NaCl. RQ-1 (A and B) and RQ-1M6 (C and D) were grown in TD medium in the absence (A and C) or presence (B and D) of exogenous trehalose. OD610, optical density at 610 nm.
- FIG. 4.
Effect of the presence of exogenous organic solutes on the cell yield of RQ-1 and RQ-1M6. RQ-1 (A) and mutant RQ-1M6 (B) were grown in TD medium containing 4, 5, and 6% NaCl. Within each group, bars represent (from left to right) TD medium without exogenous solutes and TD medium supplemented with mannosylglycerate, glycine betaine, trehalose, maltose, and glucose. OD610, optical density at 610 nm.
- FIG. 5.
Accumulation of compatible solutes by RQ-1 and RQ-1M6. RQ-1 (A) and RQ-1M6 (B) were grown in TD medium containing 3% NaCl without the addition of exogenous organic solutes (no addition) or with trehalose (Tre), maltose (Mal), or glucose (Glu). Bars represent intracellular concentrations of mannosylglycerate (▪) and trehalose (▧).
- FIG. 6.
Effect of salinity on the accumulation of solutes by RQ-1M6. RQ-1M6 was grown in TD medium without (A) or with (B) exogenous trehalose. Bars represent intracellular concentrations of mannosylglycerate (▪) and trehalose (▧).
