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POPULATION GENETICS AND EVOLUTION

Molecular Basis of the Indole-Negative Reaction in Shigella Strains: Extensive Damages to the tna Operon by Insertion Sequences

Ferdousi Rezwan, Ruiting Lan, Peter R. Reeves
Ferdousi Rezwan
1School of Molecular and Microbial Biosciences, University of Sydney
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Ruiting Lan
2School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia
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Peter R. Reeves
1School of Molecular and Microbial Biosciences, University of Sydney
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  • For correspondence: reeves@angis.usyd.edu.au
DOI: 10.1128/JB.186.21.7460-7465.2004
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    FIG. 1.

    Schematic diagram of the tna operon and genetic changes in Shigella strains. The map of the operon at the top is based on E. coli K-12. Below are shown 12 forms of the operon that can be distinguished in indole-negative Shigella serotypes. Data for D1 and S. sonnei are partly based on the incomplete genome sequences from the Sanger Centre. Blank regions indicate that PCR failed to amplify a fragment from that region while dotted lines indicate deletions with a defined end or ends. Triangles indicate IS insertions with base position marked below. Positions are given as the base pair in the gene, with negative values indicating the position relative to the start of the next gene or major feature (see the text). The name of the IS is also indicated if not IS1. Solid arrowheads at the ends of the IS indicate repeats of chromosomal DNA if present. For deletions the number of the first base is shown, with lengths in parentheses.

  • FIG. 2.
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    FIG. 2.

    Evolution of Shigella cluster 1 strains. A tree based on housekeeping gene sequences (19) is presented. Changes in housekeeping genes (19) and in the tna operon (this study) are indicated at the nodes. For example 2HK (3) means that three changes in two housekeeping genes supported that branch. Events in the tna operon are marked by the gene name with changes in parentheses. Regions that failed to amplify a product are treated as unknown events and shown as tna(unk). Changes that occurred in individual strains only are marked for the tna operon but not housekeeping genes, leading to the collapse of most external branches.

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Molecular Basis of the Indole-Negative Reaction in Shigella Strains: Extensive Damages to the tna Operon by Insertion Sequences
Ferdousi Rezwan, Ruiting Lan, Peter R. Reeves
Journal of Bacteriology Oct 2004, 186 (21) 7460-7465; DOI: 10.1128/JB.186.21.7460-7465.2004

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Molecular Basis of the Indole-Negative Reaction in Shigella Strains: Extensive Damages to the tna Operon by Insertion Sequences
Ferdousi Rezwan, Ruiting Lan, Peter R. Reeves
Journal of Bacteriology Oct 2004, 186 (21) 7460-7465; DOI: 10.1128/JB.186.21.7460-7465.2004
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  • Top
  • Article
    • ABSTRACT
    • PCR sequencing of the tna operon.
    • Indole-negative property in all Shigella strains is due to damage by IS-mediated insertion and or deletion.
    • Deletions in the tnaB gene.
    • Sequence of events in cluster 1 strains.
    • Concluding comments.
    • Nucleotide sequence accession numbers.
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
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KEYWORDS

DNA Transposable Elements
Indoles
operon
Shigella
Tryptophanase

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