Article Figures & Data
Figures
- FIG. 1.
(A and B) TEM images of wild-type W. eutropha H16 cells at 4 h (A) and 24 h (B), showing dark-stained structures (arrows). Bar, 500 nm for both panels A and B. (C) TEM image of ΔphaR W. eutropha H16 grown in TSB for 4 h, showing localization of small granules. Bar, 1.9 μm.
- FIG. 2.
TEM images of consecutive serial sections of wild-type strain W. eutropha H16 grown in TSB for 4 h. Bar, 2 μm.
- FIG. 3.
(A) Profile of PHB accumulation by wild-type strain W. eutropha H16 in TSB. (B) Expression of PhaC, PhaP, PhaR, and PhaZ1a in wild-type W. eutropha from 0 to 48 h in TSB. Note that the scale for PhaP on the right is different from the scale for the other proteins on the left. (C) Western blots of PhaC, PhaP, PhaR, and PhaZ1a from crude extracts of wild-type W. eutropha. Western blots compared to standards were used to generate the data in panel B.
- FIG. 4.
Western blot of oligomer hydrolase for the wild-type W. eutropha H16 strain. MW, molecular weight.
Tables
- TABLE 1.
In vivo concentrations and numbers of molecules of PhaC, PhaP, PhaR, and PhaZ1a in wild-type W. eutropha strain H16 at 4 and 24 h in TSB, assuming that all proteins were soluble
Protein 4 h in TSB 24 h in TSB Concn (μM) No. of molecules per cell Concn (μM) No. of molecules per cell PhaC 0.3-0.6 3.3 × 102 2.6 1.4 × 103 PhaP 36-72 3.9 × 104 48 2.6 × 104 PhaR 1-2 1.1 × 103 4.5 2.4 × 103 PhaZ1a 1.4-3 1.5 × 103 3.2 1.8 × 103 - TABLE 2.
Protein coverage of the granule surface at 4 h
Time (h) No. of PhaC molecules No. of PhaP molecules Theoreticala Expt 1b %c Theoreticala Expt 1b %c 4 2.5 × 104-5.1 × 104 3.3 × 102 0.8-1.6 0.7 × 105-1.4 × 105 3.9 × 104 27-54 24 2.7 × 104 1.4 × 103 5.5 7.4 × 104 2.6 × 104 35 ↵ a Theoretical number of protein molecules covering the total surface area of granules per cell. Note that for 4 h, a range is given due to the complications associated with cell diviosn; the total surface area of granules in a freshly divided cell is one-half that in an elongated cell before cell division.
↵ b Experimental number of protein molecules covering the total surface area of granules per cell.
↵ c Experimental/theoretical × 100.
