Article Figures & Data
Figures
- FIG. 1.
Transmission electron microscopy of the meningococcal cell wall from the wild type and the L91543/NMB0595 mutant. (a and b) OsO4-uranyl acetate staining of L91543/NMB0595 cells (a) and wild-type L91543 cells (b) before the cells were sectioned. (c) Wild-type L91543 cells sectioned prior to staining.
- FIG. 2.
Growth of the wild-type and NMB0595 mutant strains on solid media with two concentrations of magnesium. The meningococcal strains were grown overnight on peptone agar plates supplemented with either 50 mM (a) or 1 mM (b) magnesium chloride. In order to better visualize colony growth, the colonies were exposed to an oxidase reagent to increase the contrast between the growth and the agar before the plates were photographed. Quadrant 1, L91543/NMB0595 mutant; quadrant 2, wild-type strain L91543; quadrant 3, M96255789/NMB0595 mutant; quadrant 4, wild-type strain M96255789.
- FIG. 3.
Magnesium-dependent gene expression: frequency plot of normalized expression ratios for all meningococcal genes for wild-type strain L91543 (WT) and L91543/NMB0595 mutant (NMB0595) cells grown with 20 mM and 2 mM magnesium.
- FIG. 4.
Functional classification of NMB0595-deregulated and magnesium-regulated genes. The fractions of genes identified as deregulated in the L91543/NMB0595 mutant grown on blood agar (a) and differentially expressed in wild-type L91543 cells grown in 20 mM magnesium compared to cells grown in 2 mM magnesium (b) were plotted for each functional category. The numbers in parentheses indicate the total number of genes in each functional category. TCA, tricarboxylic acid. (c) Comparison of genes deregulated in L91543/NMB0595 cells grown on blood agar to lists of genes that were found to be regulated upon exposure of meningococci to either HEp-2 epithelial cells or HBMEC (8), human bronchial epithelial cell line 16HBE14 (12), human serum (31), iron (13), or heat shock (18) or identified as genes that are involved in virulence by transposon mutagenesis (47). For each graph, the fraction of expected genes can be estimated by examining the “all genes” and “hypotheticals” categories.
- FIG. 5.
In silico model of NMB0595. The model was generated as described previously (29) and was color coded according to a numerically weighted hydrophobicity scale for each amino acid, as shown in the color bar at the bottom (single-letter code).
Additional Files
Supplemental material
Files in this Data Supplement:
- Supplemental file 1 -
Table S2. Genes significantly regulated by magnesium.
PDF document, 63K. - Supplemental file 2 -
Table S1. NMB0595-deregulated genes for strains grown on blood agar plates.
PDF document, 134K.
- Supplemental file 1 -
Table S2. Genes significantly regulated by magnesium.
