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Journal of Bacteriology
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PHYSIOLOGY AND METABOLISM

Function of the Cytochrome bc1-aa3 Branch of the Respiratory Network in Mycobacteria and Network Adaptation Occurring in Response to Its Disruption

Limenako G. Matsoso, Bavesh D. Kana, Paul K. Crellin, David J. Lea-Smith, Assunta Pelosi, David Powell, Stephanie S. Dawes, Harvey Rubin, Ross L. Coppel, Valerie Mizrahi
Limenako G. Matsoso
1MRC/NHLS/WITS Molecular Mycobacteriology Research Unit, DST/NRF Centre of Excellence in Biomedical TB Research, School of Pathology, National Health Laboratory Service, and University of the Witwatersrand, Johannesburg, South Africa
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Bavesh D. Kana
1MRC/NHLS/WITS Molecular Mycobacteriology Research Unit, DST/NRF Centre of Excellence in Biomedical TB Research, School of Pathology, National Health Laboratory Service, and University of the Witwatersrand, Johannesburg, South Africa
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Paul K. Crellin
2Department of Microbiology, Monash University, and ARC Centre for Structural and Functional Microbial Genomics, Monash, Australia
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David J. Lea-Smith
2Department of Microbiology, Monash University, and ARC Centre for Structural and Functional Microbial Genomics, Monash, Australia
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Assunta Pelosi
2Department of Microbiology, Monash University, and ARC Centre for Structural and Functional Microbial Genomics, Monash, Australia
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David Powell
3Department of Computer Science and Software Engineering, Monash University, and the Victorian Bioinformatics Consortium, Monash, Australia
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Stephanie S. Dawes
1MRC/NHLS/WITS Molecular Mycobacteriology Research Unit, DST/NRF Centre of Excellence in Biomedical TB Research, School of Pathology, National Health Laboratory Service, and University of the Witwatersrand, Johannesburg, South Africa
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Harvey Rubin
4Division of Infectious Diseases, Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104
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Ross L. Coppel
2Department of Microbiology, Monash University, and ARC Centre for Structural and Functional Microbial Genomics, Monash, Australia
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Valerie Mizrahi
1MRC/NHLS/WITS Molecular Mycobacteriology Research Unit, DST/NRF Centre of Excellence in Biomedical TB Research, School of Pathology, National Health Laboratory Service, and University of the Witwatersrand, Johannesburg, South Africa
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  • For correspondence: mizrahiv@pathology.wits.ac.za
DOI: 10.1128/JB.187.18.6300-6308.2005
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ABSTRACT

The aerobic electron transport chain in Mycobacterium smegmatis can terminate in one of three possible terminal oxidase complexes. The structure and function of the electron transport pathway leading from the menaquinol-menaquinone pool to the cytochrome bc1 complex and terminating in the aa3-type cytochrome c oxidase was characterized. M. smegmatis strains with mutations in the bc1 complex and in subunit II of cyctochome c oxidase were found to be profoundly growth impaired, confirming the importance of this respiratory pathway for mycobacterial growth under aerobic conditions. Disruption of this pathway resulted in an adaptation of the respiratory network that is characterized by a marked up-regulation of cydAB, which encodes the bioenergetically less efficient and microaerobically induced cytochrome bd-type menaquinol oxidase that is required for the growth of M. smegmatis under O2-limiting conditions. Further insights into the adaptation of this organism to rerouting of the electron flux through the branch terminating in the bd-type oxidase were revealed by expression profiling of the bc1-deficient mutant strain using a partial-genome microarray of M. smegmatis that is enriched in essential genes. Although the expression profile was indicative of an increase in the reduced state of the respiratory chain, blockage of the bc1-aa3 pathway did not induce the sentinel genes of M. smegmatis that are induced by oxygen starvation and are regulated by the DosR two-component regulator.

  • Copyright © 2005 American Society for Microbiology
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Function of the Cytochrome bc1-aa3 Branch of the Respiratory Network in Mycobacteria and Network Adaptation Occurring in Response to Its Disruption
Limenako G. Matsoso, Bavesh D. Kana, Paul K. Crellin, David J. Lea-Smith, Assunta Pelosi, David Powell, Stephanie S. Dawes, Harvey Rubin, Ross L. Coppel, Valerie Mizrahi
Journal of Bacteriology Sep 2005, 187 (18) 6300-6308; DOI: 10.1128/JB.187.18.6300-6308.2005

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Function of the Cytochrome bc1-aa3 Branch of the Respiratory Network in Mycobacteria and Network Adaptation Occurring in Response to Its Disruption
Limenako G. Matsoso, Bavesh D. Kana, Paul K. Crellin, David J. Lea-Smith, Assunta Pelosi, David Powell, Stephanie S. Dawes, Harvey Rubin, Ross L. Coppel, Valerie Mizrahi
Journal of Bacteriology Sep 2005, 187 (18) 6300-6308; DOI: 10.1128/JB.187.18.6300-6308.2005
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KEYWORDS

Electron Transport Chain Complex Proteins
Electron Transport Complex III
Mycobacterium smegmatis

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