Activation of the phz Operon of Pseudomonas fluorescens 2-79 Requires the LuxR Homolog PhzR, N-(3-OH-Hexanoyl)-l-Homoserine Lactone Produced by the LuxI Homolog PhzI, and a cis-Acting phz Box

Bacterial strains and growth conditions.The bacterial strains and plasmids used in this study are described in Table 1. All E. coli strains were grown in Luria-Bertani (LB) medium at 37°C unless stated otherwise. Pseudomonas fluorescens strains 2-79 and 1855 were grown in King's medium B (23), ABM minimal medium (6), and LB medium at 28°C. The bioreporter strains, Agrobacterium tumefaciens NTL4(pZLR4) (4) and Chromobacterium violaceum CV026blu (30), were grown at 28°C in ABM and LB media, respectively. The P. fluorescens 1855 indicator strain, which harbors pSF105 and pSF107 (Table 1; see also below) was grown in ABM medium at 28°C. When required, antibiotics were added to the following final concentrations, in μg per ml: carbenicillin, 100 or 200; streptomycin or spectinomycin, 100; kanamycin, 50; gentamicin, 15 or 50; rifampin, 75; and tetracycline, 12.5 or 25.

DNA manipulations and analysis.Standard methods were used for plasmid DNA isolation, restriction enzyme digestion, agarose gel electrophoresis, ligation, and transformation (3). Plasmids were mobilized into P. fluorescens isolates using E. coli strains S17-1 and S17-1λpir as donors (Table 1). DNA was sequenced by using an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Foster City, CA), and sequence data were analyzed with the OMIGA 2.0 software package (Accelrys, San Diego, CA).

Plasmid constructions.A 1.67-kb BglII fragment containing the phzI and phzR genes of P. fluorescens 2-7 in their native configurations was subcloned from pKS-BIIB (D. V. Mavrodi, unpublished data) into the BamHI site of pUCP22, a Pseudomonas-E. coli shuttle vector (Table 1), in both orientations, giving pUCPIR and pUCPRI. The phzR gene alone was cloned as a 789-bp PCR amplicon containing the entire activator gene and its ribosome binding site from pPHZ108A (27) (Table 1) between the SmaI and BamHI sites (restriction sites were part of the PCR primers) of pUCP22 to yield pUCPR. The two regulatory genes were also cloned separately into broad-host-range expression vectors as follows. Amplicons of phzR and phzI, produced by PCR from pPHZ108A, were cloned in pBluescript II SK (Stratagene, La Jolla, CA) to give plasmids pSF101 and pSF102, respectively. The phzR gene was excised from pSF101 and cloned between the NdeI and BamHI sites (sites were generated during PCR amplification) in the broad-host-range expression vector pZLQ (26), yielding pSF105. In this plasmid, which also codes for lacI^q, phzR is under the control of the trc promoter. The phzI gene from pSF102 was excised by digestion with EcoRI and HindIII and ligated into pDLB4 (24) to yield pSF106. In this vector, phzI is under the control of P_BAD, and its expression is inducible with arabinose. All constructs were confirmed by DNA sequence analysis.

The PhzR-dependent promoter-reporter plasmid pSF107 was constructed by amplifying the intergenic region between the divergently oriented phzR and phzA genes of strain 2-79 using as a template pPHZ108A. The resultant 379-bp fragment, with a BamHI site at the phzA end and EcoRI and SmaI sites at the phzR end, was cloned into pBluescript II SK, producing pSF103. The BamHI-SmaI fragment was excised from pSF103 and ligated into the promoter selection vector pRG970b (51) (Table 1), yielding pSF107. In this construct, transcription toward phzA drives expression of lacZ while transcription toward phzR drives expression of uidA.

The lux box-like sequence located in the divergent promoter region was disrupted by digesting pSF103 with BglII, which cuts in the middle of the box. The ends were filled in with Klenow fragment. Following self-ligation, plasmids isolated from transformants were tested for the loss of the BglII site by restriction digestion. The disruption was confirmed by sequence analysis, and the mutated bidirectional promoter was cloned into pRG970b, giving rise to pSF108.

Gene replacement mutagenesis.Mutants of P. fluorescens 2-79 were constructed using the gene replacement strategy previously described by Schweizer (42). To generate strain 2-79IR, which lacks the phzI and phzR genes (Fig. 1A), a 6.3-kb PstI fragment of pPHZ108A, which contains the phz operon together with the phzI and phzR genes of strain 2-79 (Fig. 1A), was subcloned into pBluescript II KS, yielding pKS-Pst6.3. The plasmid was digested with BglII, thereby removing a 1.67-kb fragment containing the phzI and phzR genes, and the ends were polished with the Klenow fragment. The 1.67-kb fragment was replaced with a 2.5-kb PvuII fragment encoding a tetracycline resistance gene from pALTER-Ex1 (Promega Corp., Madison, WI). The interrupted insert was subcloned into pNOT19 (42), yielding pNOT-Pst6.3-Tc^r, which was digested with NotI and ligated with a 5.3-kb sacB cassette from pMOB3 (Table 1). The resulting plasmid, pNOT-Pst6.3-Tc^r-MOB, was mobilized into P. fluorescens 2-79, and transconjugants were selected on LB agar supplemented with rifampin and tetracycline. Following selection for double crossovers on LB agar supplemented with sucrose (28), isolates were screened phenotypically for the absence of the plasmid-borne bla and sacB markers and the presence of tetA. The deletion replacement event was confirmed by PCR analysis.

To construct strain 2-79Z containing lacZ fused to phzD (Fig. 1), a 4.3-kb fragment containing the phzCDE interval was subcloned from pT7-5CDE (27) into pEX18Tc (20) (Table 1), yielding pEX18Tc-CDE. The plasmid was treated sequentially with NcoI, Klenow fragment, and PstI and ligated with a 3.1-kb fragment from pMC1871 containing a promoterless lacZ gene, giving pEX18Tc-CDE-lacZ. This construct contains a translational fusion of lacZ to phzD. pEX18Tc-CDE-lacZ was introduced into strain 2-79 by electroporation, and transformants were selected on LB agar supplemented with tetracycline. Double crossovers were selected, and mutations were verified by PCR as described above.

To construct strain 2-79IZ, a phzI disruption mutant of strain 2-79Z (Fig. 1), a 515-bp 5′ segment of phzI was amplified by PCR and cloned as a KpnI-BamHI fragment (sites were part of the primers) into pEX18Gm (20) to give pEX18Gm-I. A second, ca. 2.4-kb fragment containing a 3′ segment of phzI, as well as phzR, phzA, and phzB, was amplified from pPHZ108A by PCR and cloned into pEX18Gm-I between the BamHI and XbaI sites, yielding pEX18Gm-I-Bam. These manipulations resulted in introduction of a unique BamHI site within the phzI gene. A 2.5-kb PvuII fragment containing the tet cassette from pALTER-Ex1 was cloned into the BamHI site of phzI in pEX18Gm-I-Bam, yielding pEX18Gm-I-Tc^r. This plasmid was introduced into strain 2-79Z by electroporation, and double-crossover events were selected for and confirmed as described above.

Primer extension analysis.Transcription start sites were mapped by primer extension essentially as described by Ausubel et al. (3). Total RNA was isolated from cultures of strain 2-79 using an RNeasy Midi Kit (QIAGEN). The transcriptional start site of phzA was determined using primer PHSPA (5′-CGC GGT TTT TTC GGC GAA GTT C-3′ [positions 1872 through 1893 in GenBank entry L48616 ]). The transcriptional start site of phzR was determined using primer PHSPR (5′-CTC CTC CAT GTT CAT GGC TTG TGC-3′ [positions 1368 through 1391 in GenBank entry L48616 ]) (27). Oligonucleotides end labeled with [γ-³²P]ATP were mixed with 15 μg of RNA at 65°C in a buffer containing 50 mM Tris-HCl, pH 8.3, and 45 mM KCl and annealed by slowly cooling them to room temperature. Primer extension was performed at 42°C with SuperScript II RNase H⁻ reverse transcriptase (Life Technologies, Inc.). Extension products were separated on denaturing 6% polyacrylamide-8 M urea gels next to sequencing ladders generated from pKS-Pst6.3 (Table 1) with the same primers using Sequenase version 2.0 (Amersham Biosciences Corp.).

Analysis of phenazines.Cultures of P. fluorescens 2-79 and its mutants were grown for 24 h at 28°C in LB medium supplemented with 0.2% glucose and antibiotics as appropriate. The cells were removed by centrifugation, and the culture supernatants were acidified with trifluoroacetic acid and extracted twice with ethyl acetate. Organic phases were pooled and evaporated to dryness, and residues were redissolved in 35% acetonitrile-0.1% trifluoroacetic acid. PCA was resolved by high-performance liquid chromatography (HPLC) on a NOVA-PAK C₁₈ reverse-phase Radial-PAK cartridge (4 μm; 8 by 100 mm) (Waters Corp., Milford, MA) with a linear gradient (8 to 100%) of acetonitrile in 0.1% trifluoroacetic acid at a flow rate of 1.0 ml per min using a Waters HPLC system. Elution profiles were monitored at the absorbance maxima (248 nm and 366 nm) characteristic of PCA in the solvent system using a Waters 990 photodiode array detector. Reference samples of PCA were purchased from Color Your Enzyme (Bath, Ontario, Canada).

Extraction of acyl-homoserine lactones from culture supernatants.Cultures of strain 2-79 and its mutants were grown in King's medium B for 48 h at 28°C. Cultures of Escherichia coli and P. fluorescens 1855 expressing the arabinose-inducible phzI gene of strain 2-79 in pSF106 were grown in LB and ABM media, respectively, to an optical density at 600 nm of 0.4. Arabinose was added to a final concentration of 0.3%, and incubation was continued for another 15 to 18 h at 28°C. The bacteria were removed by centrifugation, the supernatants were extracted twice with equal volumes of ethyl acetate or dichloromethane, the organic phases were collected and pooled, and residual water was removed by addition of anhydrous sodium sulfate. The extracts were taken to dryness under vacuum, and the residues were redissolved in volumes of HPLC grade ethyl acetate or dichloromethane sufficient to yield concentration factors of 100 to 1,000.

Synthesis of acyl-homoserine lactone standards.The N-(3-hydroxy-hexanoyl)-l-HSL and N-(3-hydroxy-octanoyl)-l-HSL standards were synthesized by the Reformatsky reaction of hexanal or butanal with ethyl bromoacetate in the presence of activated zinc (16, 46). This reaction results in the formation of ethyl 3-hydroxy-octanoate (with hexanal) or ethyl 3-hydroxy-hexanoate (with butanal), each of which was subsequently hydrolyzed by lithium hydroxide in tetrahydrofuran-water (1:1 [vol/vol]) and condensed with l-homoserine lactone (Sigma-Aldrich) using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride-dimethylaminopyridine. The structures of the synthetic acyl-HSLs were confirmed by analytical thin-layer chromatography (TLC) and by ¹H nuclear magnetic resonance on a Varian Gemini 300-, Inova 400-, or Inova 500-MHz spectrometer. ¹H nuclear magnetic resonance shifts were referenced to tetramethylsilane (0.0 ppm). N-(Hexanoyl)-l-HSL was purchased from Sigma-Aldrich.

Separation and visualization of acyl-homoserine lactones.Two- to 5-μl volumes of samples to be analyzed were applied to C₁₈ reverse-phase TLC plates and developed in methanol-water (60:40) as described previously (4, 45). The plates were dried, overlaid with appropriate indicator strains, incubated, and examined as described previously (15, 45).

Quantification of acyl-HSLs in culture supernatants.Serial dilutions of ethyl acetate extracts of culture supernatants from strain 2-79 were chromatographed on TLC plates alongside dilution series of standard acyl-HSLs of known concentrations. Following development, the TLC plates were overlaid with cultures of the A. tumefaciens or C. violaceum indicator strain and incubated at 28°C. The diameters of the spots of different AHLs present in the mixture were measured and compared with the spots of standard acyl-HSLs of known concentrations chromatographed on the same plate as previously described (15, 45).

β-Galactosidase and β-glucuronidase assays.β-Galactosidase or β-glucuronidase activities were assessed qualitatively by patching cultures onto solid ABM or LB media containing X-Gal (5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside) or 5-bromo-4-chloro-3-indolyl-β-d-glucuronoside, respectively. Colony color and intensity were visually assessed after 18 to 36 h of growth at 28°C. β-Galactosidase activity was quantified according to the method of Miller (31), while β-glucuronidase activity was quantified as described by Jefferson et al. (22).

Production of PCA by P. fluorescens 2-79 requires PhzR and PhzI.The phz operon of P. fluorescens 2-79 is preceded by two genes, phzR and phzI (Fig. 1A), the organization and sequence of which are highly conserved relative to homologs forming a quorum-sensing regulatory pair that activates expression of the phz operon in other soil pseudomonads (8, 34, 56). To determine if phzI and phzR are required for expression of the phz operon in strain 2-79, we replaced the two genes with a tet cassette (Fig. 1A). Whereas the parent strain produced PCA as detected by biological activity and chemical analysis, the deletion mutant, 2-79IR, failed to produce any detectable PCA (Fig. 1B and data not shown). Production of PCA was partially restored to strain 2-79IR by complementation with either pUCPRI or pUCPIR (Table 1), both of which carry phzI and phzR (data not shown).

When pUCPR, expressing only phzR (Table 1), was introduced into strain 2-79IR, the mutant failed to produce detectable levels of PCA (data not shown). However, when the culture was supplemented with an ethyl acetate extract of a culture supernatant of strain 2-79Z, the complemented mutant produced small but detectable amounts of the antibiotic (data not shown). Addition of the ethyl acetate extract to cultures of strain 2-79IR lacking the phzR clone did not restore production of PCA (data not shown). Combined, the data suggest that production of PCA by P. fluorescens 2-79 is controlled by PhzR and that this LuxR homolog requires an organic-soluble autoinducer likely produced by PhzI.

The phzI gene of strain 2-79 codes for the synthesis of 3-hydroxy- and alkanoyl-acyl-homoserine lactones.The phz quorum-sensing systems of P. aureofaciens 30-84 and P. chlororaphis PCL1391 respond to C6-HSL (8, 57). On the other hand, we previously reported that strain 2-79 produces six signals: C6- and C8-HSL and three 3-OH derivatives with side chains of 6, 8, and 10 carbons (45). By mass spectrometry and chemical synthesis, we have since identified the sixth signal as the rare seven-carbon species N-(3-hydroxy-heptanoyl)-l-HSL (Fig. 2) (reference 12 and data not shown).

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FIG. 2.

Deletion of phzI results in the loss of production of all acyl-HSLs normally synthesized by P. fluorescens 2-79. Ethyl acetate extracts of supernatants from cultures of wild-type P. fluorescens 2-79 (lanes 1), the phzD::lacZ mutant, 2-79Z (lanes 2), and the phzI deletion mutant, 2-79ZI (lanes 3), each grown in King's medium B to late exponential phase, were subjected to thin-layer chromatography, and the developed plates were overlaid with cultures of the A. tumefaciens (A) or C. violaceum (B) bioreporter, all as described in Materials and Methods. The position of migration of each active acyl-HSL component, identified as described previously (45), is indicated at the margin of each panel.

We assessed the role of the phzI gene in the production of these signals by examining culture supernatants of P. fluorescens 2-79IZ, in which phzI is deleted, for quormone content. As assessed by TLC, extracts from supernatants of late-exponential-phase cultures of strain 2-79IZ did not contain any signals detectable using either the A. tumefaciens or C. violaceum reporter (Fig. 2A and B, lanes 3).

It is possible that PhzI produces C6-HSL and that this signal, interacting with PhzR, activates another quorum-sensing system in strain 2-79 responsible for production of the 3-hydroxy-HSL signals. To test this hypothesis, we analyzed the acyl-HSL complement produced by heterologous bacteria expressing the phzI gene of strain 2-79 cloned in pSF106 (Table 1). Whether expressed in E. coli DH5α or in P. fluorescens 1855, neither of which produce their own acyl-HSLs (4, 45), the cloned phzI gene directed the synthesis of all six of the 3-hydroxy- and alkanoyl-acyl-HSLs characteristically produced by strain 2-79 (Fig. 3A and B). Strains harboring the pDLB4 vector alone did not produce detectable amounts of any active compound.

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FIG. 3.

The phzI gene of P. fluorescens 2-79 confers production of all six acyl-HSLs. Ethyl acetate extracts of culture supernatants of strains harboring the cloned phzI gene in pSF106 or the vector pDLB4 were chromatographed, and the plates were overlaid with the A. tumefaciens (A) or C. violaceum (B) reporter strain as described in Materials and Methods. The lanes contain extracts from cultures of (1) P. fluorescens 2-79, (2) E. coli DH5α(pDLB4), (3) DH5α(pSF106), (4) P. fluorescens 1855(pDLB4), and (5) 1855(pSF106). Lane 6 contains a sample of pure N-hexanoyl-HSL standard. The locations and identities of the six acyl-HSLs are indicated at the margin of each panel.

We quantified the amounts of the major 3-OH and alkanoyl forms present in culture supernatants of strain 2-79 as described in Materials and Methods. In one such sample taken from a culture grown in minimal medium to late exponential phase, 3-OH-C6-HSL, at a concentration of 1.3 μM, dominated the mixture, while C6-HSL accumulated to 0.075 μM. 3-OH-C8-HSL, present at 0.04 μM, was the least abundant of the three species examined. Although the absolute amounts of each species varied as a function of culture phase and medium, the relative amounts of each of the tested species remained the same in all samples tested (data not shown). Moreover, the relative amounts of each species did not differ in samples prepared by extraction with ethyl acetate or dichloromethane (data not shown).

PhzR recognizes 3-OH-C6-HSL with the highest sensitivity.To assess how PhzR-dependent regulation in strain 2-79 responds to the different signals, we constructed a reporter system composed of the cloned phzR gene on pSF105 and the phzR-phzA divergent dual promoter region fused between the oppositely oriented uidA and lacZ reporters on pSF107 (Table 1 and Fig. 4A). The two plasmids were introduced into P. fluorescens 1855, which neither synthesizes detectable acyl-HSLs nor produces detectable amounts of β-galactosidase or β-glucuronidase (data not shown). When grown in AB minimal medium to late exponential phase, the lacZ and the uidA reporter fusions were expressed at low but detectable levels (Table 2). However when grown in medium supplemented with an ethyl acetate extract of a culture supernatant of strain 2-79, the phzA reporter was induced almost 30-fold compared to cells grown without extract (Table 2). The phzR reporter was induced about twofold by the ethyl acetate extract (Table 2). Although low, this extract-dependent autoactivation of phzR by PhzR was observed consistently. In qualitative assays on medium containing X-Gal, induction of both activities required PhzR; strains lacking pSF105 exhibited no significant reporter activity even when cultured with the acyl-HSL (data not shown).

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FIG. 4.

PhzR responds with greatest sensitivity to N-(3-OH-hexanoyl)-l-homoserine lactone. The intergenic region between phzA and phzI was cloned in the promoter vector pRG970b to form pSF107 as described in Materials and Methods. In this vector, the divergent promoter region forms reporter fusions to lacZ and uidA (A). P. fluorescens 1855(pSF107), also harboring pSF105, which codes for PhzR, was grown for 4 hours in ABM medium containing different concentrations of (□) N-(3-OH-hexanoyl)-HSL, (⋄) N-hexanoyl-HSL, or (○) N-(3-OH-octanoyl)-HSL. The cells were assayed for β-galactosidase activity from the phzA::lacZ reporter, expressed as Miller units (B), as described in Materials and Methods. The experiment was repeated twice, and a representative data set is shown. AI, acyl-HSL autoinducer.

When tested with increasing concentrations of pure samples, the phzA::lacZ reporter of pSF107 exhibited the greatest sensitivity to and was induced to the highest levels by 3-OH-C6-HSL (Fig. 4B). The reporter also responded to 3-OH-C8-HSL and to C6-HSL but required concentrations of these two acyl-HSLs almost 10-fold higher than that of the 3-OH-C6 signal. Moreover, even when grown with saturating concentrations of C6-HSL or 3-OH-C8-HSL, the phzA::lacZ reporter yielded levels of maximum activation only about half that observed in cultures grown with 3-OH-C6-HSL (Fig. 4B).

To assess the response of PhzR to the mixture of acyl-HSLs produced by strain 2-79, we chromatographed a sample of an ethyl acetate extract of a culture supernatant from the wild-type phenazine producer and overlaid the developed TLC plate with a mixture of the C. violaceum and P. fluorescens 1855 phz reporter strains (Fig. 5). In this mixed indicator system, the C. violaceum reporter, which recognizes C4 and C6 alkanoyl-HSLs (4, 30, 45), produces a purple pigment while the P. fluorescens phzA::lacZ indicator produces indol from hydrolysis of X-Gal, each in response to the acyl-HSLs to which it can react. The C. violaceum reporter in the mixture clearly responded to the C6-HSL, both in pure form (Fig. 5, lanes 4 and 5), and that present in the ethyl acetate extract (Fig. 5, lane 1). Moreover, of the six acyl-HSLs present in the sample, only C6-HSL was detectable by C. violaceum. The P. fluorescens phzA::lacZ reporter responded to only one standard, 3-OH-C6-HSL, and only to the 3-OH-C6 species present in the extract from the culture supernatant (Fig. 5, lanes 1, 3, and 5). This reporter did not respond to the alkanoyl quormone standard (Fig. 5, lane 4) or to the amount of C6-HSL present in the extract of the culture supernatant from strain 2-79 (Fig. 5, lane 1), nor did the P. fluorescens reporter respond to any of the other 3-OH- or alkanoyl-acyl-HSLs present in the culture extracts (Fig. 5, lane 1).

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FIG. 5.

PhzR responds only to the 3-OH-hexanoyl-HSL present in culture supernatants of P. fluorescens 2-79. An ethyl acetate extract of a culture supernatant of P. fluorescens 2-79 grown to late exponential phase in King's medium B, as well as several standard compounds, was chromatographed, and the TLC plate was overlaid with a mixture of P. fluorescens 1855(pSF105, pSF107) and C. violaceum CV026blu as described in Materials and Methods. The blue spots represent acyl-HSLs detected by the P. fluorescens phzA::lacZ reporter, while the purple spots correspond to species detected by C. violaceum. Lanes contain samples of (1) extract from a culture supernatant of P. fluorescens 2-79 and pure standards of (2) N-(3-OH-octanoyl)-HSL, (3) N-(3-OH-hexanoyl)-HSL, (4) N-hexanoyl-HSL, and (5) a mixture of N-(3-OH-hexanoyl)-HSL and N-hexanoyl-HSL.

Other acyl-HSLs do not significantly influence activation of the phzA lacZ reporter by PhzR in response to 3-OH-C6-HSL.We considered the possibility that one or more of the acyl-HSLs produced by P. fluorescens 2-79 exert a modulating activity on PhzR-mediated gene activation. When grown in medium supplemented with only one of the three most abundant acyl-HSLs produced by strain 2-79, the P. fluorescens 1855 phzA::lacZ reporter responded with highest sensitivity to the 3-OH-C6 species and with less sensitivity to C6-HSL and 3-OH-C8-HSL (Fig. 6). Growth in medium supplemented with mixtures containing equal molar amounts of C6-HSL or 3-OH-C8-HSL, along with 3-OH-C6-HSL, gave slightly higher levels of response than cultures grown with only 3-OH-C6-HSL. A culture supplemented with equal molar amounts of all three acyl-HSLs induced the phzA::lacZ reporter to levels 30 to 40% higher than that seen in cultures incubated with 3-OH-C6-HSL alone or with mixtures of 3-OH-C6-HSL and one of the other two components (Fig. 6). Cultures incubated with a mixture of C6-HSL and 3-OH-C8-HSL but containing no 3-OH-C6-HSL induced the reporter to low levels similar to that observed in cultures grown with C6-HSL or 3-OH-C8-HSL (Fig. 6).

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FIG. 6.

Noncognate acyl-HSLs have little effect on activation of PhzR by N-(3-OH-hexanoyl)-HSL. Individual cultures of P. fluorescens 1855(pSF105, pSF107), grown for 4 h in ABM medium with a single acyl-HSL or with mixtures of component acyl-HSLs, all at final concentrations of 500 nM, were assayed for β-galactosidase activities from phzA::lacZ as described in Materials and Methods. The cultures were supplemented with (1) N-(3-OH-hexanoyl)-HSL, (2) N-hexanoyl-HSL, (3) N-(3-OH-octanoyl)-HSL, (4) N-(3-OH-hexanoyl)-HSL and N-hexanoyl-HSL, (5) N-hexanoyl-HSL and N-(3-OH-octanoyl)-HSL, (6) N-(3-OH-hexanoyl)-HSL and N-(3-OH-octanoyl)-HSL, or (7) N-(3-OH-hexanoyl)-HSL, N-hexanoyl-HSL, and N-(3-OH-octanoyl)-HSL.

Transcriptional start sites for phzA and phzR.Using primer extension analysis, we located the transcriptional start site for the phz operon to a G residue positioned 111 bp upstream of the translational initiation codon of phzA (Fig. 7B). This site is located 10 bp downstream from the center of a hexamer, 5′-TATAAA-3′, with a good sequence match to that of the consensus −10 promoter element (Fig. 7A). We also located the transcriptional start site of the divergently oriented phzR gene to a G residue 63 bp upstream of the putative translational start codon (Fig. 7C). This site is located downstream of properly spaced hexamers showing only weak relatedness to consensus −10 and −35 elements (Fig. 7A).

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FIG. 7.

The divergent promoter region between phzR and the phz operon of P. fluorescens 2-79 contains cis-acting sites required for PhzR-mediated activation. The nucleotide sequence of the 379-bp region between phzR and phzA (A) contains putative −10 and −35 elements and ribosomal binding sites (RBS) as marked. The transcriptional start sites, shown as arrows labeled +1, were determined by primer extension analysis for phzA (B) and phzR (C) as described in Materials and Methods. The region also contains an 18-bp almost perfect inverted-repeat (phz box) that is related to the lux box sequence of Vibrio fischeri and also to inverted repeats located in the promoter regions of the phz operons of P. aureofaciens 30-84 and P. chlororaphis PCL1391 (D).

An intact phz box is required for PhzR-mediated activation of the phzA and phzR genes.The intergenic promoter region also contains an 18-bp almost-perfect inverted repeat that resembles the lux box family of transcription factor binding sites (Fig. 7A and D). This repeat is situated upstream and immediately adjacent to the probable −35 element of the phzA promoter and approximately 120 bp upstream from the putative −35 element of the phzR promoter (Fig. 7A). We tested the requirement of this site for transcription by constructing a 4-bp insertion that duplicates the central GATC of the putative phz box sequence present in our standard reporter plasmid pSF107 (Table 1). Cultures of the strains harboring the wild-type and mutant reporter clones grown in the absence of an acyl-HSL signal expressed the phzA::lacZ and phzR::uidA fusions at low basal levels (Table 3). As expected, the strain harboring the wild-type promoter segment strongly expressed the lacZ reporter when grown with the acyl-HSL signal. However, in the reporter harboring the insertion mutant, expression of the phzA::lacZ fusion remained at basal levels even when the strain was grown with 3-OH-C6-HSL (Table 3). Similarly, the phzR::uidA fusion was induced almost twofold in response to the 3-OH-C6 signal in the strain harboring pSF107, but expression of this reporter remained at basal levels in the strain harboring the mutant phz box sequence in pSF108 (Table 3).