Article Figures & Data
Figures
- FIG. 1.
Hybridization of genomic DNA from P. woesei to the P. furiosus DNA microarray. The data were normalized as described in Materials and Methods. The abscissa indicates P. furiosus ORFs (ORFs 1 to 2065). The bar at the top indicates the positions of P. furiosus insertion sequences.
- FIG. 2.
Growth of Pyrococcus species on 99% pure maltose. Symbols: ⧫, P. furiosus; ▴, P. woesei.
- FIG. 3.
Growth of P. woesei and P. furiosus in the presence of the NO generator RBS. The arrows indicate times at which RBS (0.9 μM) was added. Symbols: ▴, P. furiosus with RBS; ⧫, P. furiosus without RBS; ▪, P. woesei with RBS; •, P. woesei without RBS.
- FIG. 4.
Products of the Mal I region of P. furiosus and P. woesei as determined by PCR analysis. The products were analyzed on an agarose gel (0.5%, wt/vol) and were stained with ethidium bromide. The λ/HindIII-φX174/HaeIII DNA ladder was obtained from Stratagene.
- FIG. 5.
Sequences of the PCR products of the Mal I regions of P. furiosus and P. woesei. See text for details. The sequenced ORFs of the unsequenced P. woesei genome are labeled ‘PF1735’ and ‘PF1753.’ The nucleotide sequences of these two ORFs in P. woesei are identical to the nucleotide sequences of the corresponding P. furiosus ORFs.
Tables
- TABLE 1.
P. furiosus ORFs that lack homologs in the P. woesei genome based on DNA microarray and PCR analysesa
ORFb A B C InterPRO annotation ORFb A B C InterPRO annotation PF0025 * * * Glutamine amidotransferase II PF0735 No InterPRO entry PF0026 * * * DNA polymerase, beta region PF0737 No InterPRO entry PF0029 No InterPRO entry PF0738 S-Adenosylmethionine binding protein PF0030 No InterPRO entry PF0739 Bacterial regulator, AsnC/Lrp PF0031 PLP enzyme, beta subunit PF0740 * Copper-transporting ATPase PF0032 No InterPRO entry PF0742 * Ferritin and Dps PF0034 * No InterPRO entry PF0743 Coenzyme A binding domain PF0035 * ABC transporter PF0744 * ABC transporter PF0037 ARM repeat fold PF0758 * * HEPN, nucleotide-binding PF0038 * * Beta-lactamase-like PF0760 * * Transposase, IS605 OrfB PF0041 * * * Elongator protein 3/MiaB/NifB PF0762 * * No InterPRO entry PF0151 * Glycoside hydrolase, family 5 PF0763 No InterPRO entry PF0152 * * Protein of unknown function PF0764 * * * Archaeal ATPase PF0154 * * * Glutamine amidotransferase II PF0765 * 6-Phosphogluconate DH, C terminal PF0365 Protein of unknown function PF0766 * Oxidoreductase, N terminal PF0366 * * * No InterPRO entry PF0767 * Deg T aminotransferase PF0685 Protein of unknown function PF0768 * Transferase hexapeptide repeat PF0687 Transcription factor TFIIB PF0769 * Glycosyl transferase, family 2 PF0689 Prismane-like PF0770 * UTP-glucose-1-Pi transferase PF0691 * Lambda repressor-like PF0772 * DNA polymerase, beta-like PF0692 * Prismane PF0773 No InterPRO entry PF0693 SirA-like PF0775 No InterPRO entry PF0694 Flavodoxin/NO synthase PF0776 * No InterPRO entry PF0695 * Protein of unknown function PF0777 No InterPRO entry PF0697 Sugar transporter superfamily PF0781 * * Nucleotide binding protein, PINc PF0698 No InterPRO entry PF0783 * No InterPRO entry PF0701 * * No InterPRO entry PF0790 No InterPRO entry PF0702 * * Peptidase M24 PF0791 Glycosyl transferase, group 1 PF0703 No InterPRO entry PF0792 No InterPRO entry PF0704 Protein of unknown function PF0794 UDP-N-Ac gluc.-2-epimerasec PF0705 Cytochrome c biogenesis PF0795 Glycosyl transferase, group 1 PF0706 Protein of unknown function PF0796 Transferase hexapeptide repeat PF0707 4-Oxalocrotonate tautomerase PF0797 No InterPRO entry PF0708 Extrusion protein MatE PF0798 Glycosyl transferase, family 2 PF0712 No InterPRO entry PF1337 * * TENA/THI-4 protein PF0713 * Winged helix DNA binding PF1339 Protein of unknown function PF0715 Nitroreductase PF1340 Protein of unknown function PF0716 6-Phosphogluconate dehydrogenase PF1737 No InterPRO entry PF0717 S-Adenosylmethionine binding motif PF1738 Ribokinase PF0718 No InterPRO entry PF1339 Protein of unknown function PF0719 No InterPRO entry PF1740 Membrane transport component PF0720 No InterPRO entry PF1741 Membrane transport component PF0721 NADPH: flavin mononucleotide reductase PF1742 * Glycosyl transferase, group 1 PF0722 Alkyl hydroperoxide reductase PF1743 * Protein of unknown function PF0723 Iron permease FTR1 PF1744 * * * ABC transporter PF0725 Coenzyme A binding domain PF1745 * * l-Fucose isomerase PF0727 No InterPRO entry PF1746 Glycoside transferase PF0729 4Fe-4S ferredoxin PF1747 Protein of unknown function PF0730 * No InterPRO entry PF1748 * Membrane transport component PF0731 Ferritin-like PF1749 * Membrane transport component PF0732 ABC transporter PF1750 * * * ABC transporter PF0733 ABC transporter PF1751 * * Thiamine ABC transporter PF0734 No InterPRO entry a Shading indicates potential ORF clusters (in which adjacent ORFs can be on the positive or negative strand). An asterisk indicates the presence of a homolog. See text for details.
↵ b For each ORF, the presence of a homolog in P. horikoshii (A), P. abyssi (B), or T. kodakaraensis (C) is shown.
↵ c UDP-N-acetylglucosamine-4-epimerase.
