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GENETICS AND MOLECULAR BIOLOGY

The Vibrio harveyi GTPase CgtAV Is Essential and Is Associated with the 50S Ribosomal Subunit

A. E. Sikora, R. Zielke, K. Datta, J. R. Maddock
A. E. Sikora
1Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 North University, Ann Arbor, Michigan 48109
2Department of Molecular Biology, University of Gdansk, Kładki 24, 80-822 Gdansk, Poland
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R. Zielke
1Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 North University, Ann Arbor, Michigan 48109
2Department of Molecular Biology, University of Gdansk, Kładki 24, 80-822 Gdansk, Poland
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K. Datta
1Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 North University, Ann Arbor, Michigan 48109
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J. R. Maddock
1Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 North University, Ann Arbor, Michigan 48109
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  • For correspondence: maddock@umich.edu
DOI: 10.1128/JB.188.3.1205-1210.2006
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ABSTRACT

It was previously reported that unlike the other obg/cgtA GTPases, the Vibrio harveyi cgtAV is not essential. Here we show that cgtAV was not disrupted in these studies and is, in fact, essential for viability. Depletion of CgtAV did not result in cell elongation. CgtAV is associated with the large ribosomal particle. In light of our results, we predict that the V. harveyi CgtAV protein plays a similar essential role to that seen for Obg/CgtA proteins in other bacteria.

  • Copyright © 2006 American Society for Microbiology
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The Vibrio harveyi GTPase CgtAV Is Essential and Is Associated with the 50S Ribosomal Subunit
A. E. Sikora, R. Zielke, K. Datta, J. R. Maddock
Journal of Bacteriology Jan 2006, 188 (3) 1205-1210; DOI: 10.1128/JB.188.3.1205-1210.2006

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The Vibrio harveyi GTPase CgtAV Is Essential and Is Associated with the 50S Ribosomal Subunit
A. E. Sikora, R. Zielke, K. Datta, J. R. Maddock
Journal of Bacteriology Jan 2006, 188 (3) 1205-1210; DOI: 10.1128/JB.188.3.1205-1210.2006
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  • Top
  • Article
    • ABSTRACT
    • Bacterial strains, plasmids, and growth conditions.
    • The V. harveyi cgtAV gene is essential.
    • The previously reported cgtA::Tn5TpMCS mutant, BB7X, encodes a wild-type cgtAV gene.
    • Expression of the V. harveyi folA gene in E. coli results in trimethoprim resistance.
    • The V. harveyi CgtAV protein is associated with the 50S ribosomal particle.
    • Concluding remarks.
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
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KEYWORDS

Bacterial Proteins
GTP Phosphohydrolases
Monomeric GTP-Binding Proteins
Ribosomal Proteins
Vibrio

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