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ENZYMES AND PROTEINS

Two Putative c-Type Multiheme Cytochromes Required for the Expression of OmcB, an Outer Membrane Protein Essential for Optimal Fe(III) Reduction in Geobacter sulfurreducens

Byoung-Chan Kim, Xinlei Qian, Ching Leang, Maddalena V. Coppi, Derek R. Lovley
Byoung-Chan Kim
Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003
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  • For correspondence: bckim@microbio.umass.edu
Xinlei Qian
Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003
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Ching Leang
Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003
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Maddalena V. Coppi
Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003
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Derek R. Lovley
Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003
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DOI: 10.1128/JB.188.8.3138-3142.2006
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ABSTRACT

Deletion of two homologous Geobacter sulfurreducens c-type cytochrome genes, omcG and omcH, decreased the rate of Fe(III) reduction and decreased the level of an outer membrane cytochrome critical for Fe(III) reduction, OmcB, without affecting its transcription. Expression of either gene restored Fe(III) reduction and OmcB expression, suggesting functional similarity.

  • Copyright © 2006 American Society for Microbiology
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Two Putative c-Type Multiheme Cytochromes Required for the Expression of OmcB, an Outer Membrane Protein Essential for Optimal Fe(III) Reduction in Geobacter sulfurreducens
Byoung-Chan Kim, Xinlei Qian, Ching Leang, Maddalena V. Coppi, Derek R. Lovley
Journal of Bacteriology Apr 2006, 188 (8) 3138-3142; DOI: 10.1128/JB.188.8.3138-3142.2006

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Two Putative c-Type Multiheme Cytochromes Required for the Expression of OmcB, an Outer Membrane Protein Essential for Optimal Fe(III) Reduction in Geobacter sulfurreducens
Byoung-Chan Kim, Xinlei Qian, Ching Leang, Maddalena V. Coppi, Derek R. Lovley
Journal of Bacteriology Apr 2006, 188 (8) 3138-3142; DOI: 10.1128/JB.188.8.3138-3142.2006
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  • Top
  • Article
    • ABSTRACT
    • Construction and characterization of omcG, omcH, and omcA deletion mutants.
    • OmcB expression in the omcA omcH omcG::kan triple mutant and the complemented strains.
    • Implications.
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
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KEYWORDS

Bacterial Outer Membrane Proteins
Cytochromes c
Ferric Compounds
Geobacter

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