Flagellar Motility and Structure in the Hyperthermoacidophilic Archaeon Sulfolobus solfataricus

Analysis of the fla gene locus in Sulfolobus solfataricus.

The flagellum operon of the sequenced S. solfataricus P2 strain is disrupted by an insertion sequence (IS) element integrated into the coding region of the flaG gene (SSO2321 [34]). However, PCR analysis of the gene region suggested that this integration is not stable under laboratory growth conditions, as we frequently observed flagellated S. solfataricus P2 cells (Fig. 1B). Additionally, this IS element is completely absent in a related strain, S. solfataricus PBL2025 (an S. solfataricus 98/2 derivative [21]), as determined by PCR amplification and sequence analysis of the flaG gene (data not shown). The coding sequences of the flaG gene were identical in both S. solfataricus strains at the nucleotide level. The flagellum (fla) operon encodes seven open reading frames (Fig. 1A), one structural protein (FlaB), five putative accessory proteins (FlaGFHIJ), and one open reading frame (SSO2322) for which homologs can be found only in the other two available Sulfolobus genomes (12, 23). The last open reading frame is not homologous to any known flagellar accessory protein but contains a putative coiled-coil domain. To find conserved regions within the flaB promoter, sequences upstream of flaB genes obtained from the three available Sulfolobus sp. genome sequences were aligned using ClustalW (13). A 5-base-pair direct repeat was detected, starting 75 bases upstream of the translational start site and 30 bases upstream of a putative TATA box and BRE site. A direct repeat sequence was previously identified upstream of the flaB1 gene of the methane-producing archaeon Methanococcus jannaschii, which could be a transcriptional regulator binding site (38). In this organism, the induction of flagellar protein synthesis was detected at high cell densities and excess hydrogen, or low hydrogen, partial pressure (28).

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FIG. 1.

Genomic organization of the fla operon and flagellation of S. solfataricus P2. (A) Schematic representation of the S. solfataricus fla operon. An IS element disrupted the SSO2321 gene in the sequenced P2 strain (insertion site indicated by an arrowhead) but is absent in PBL2025. Genes are shown as open arrows with gene designations based on homology. The structural flagellin gene is in black, and genes with predicted accessory functions are gray. Suffixes of fla genes with identified homologies are depicted in the boxes, while locus tag (SSO) numbers are given above the boxes. SSO2322 (white arrow) is so far unique to the genus Sulfolobus. A conserved 5-base direct repeat (underlined), starting 75 bases upstream of the translational start site of flaB, is shown. The consensus sequence was derived from an alignment of flaB promoter sequences from three sequenced Sulfolobus species. Nucleotide base codes are as follows: Y, C or T; N, any nucleotide. (B) Transmission electron microscopy image of a negatively stained flagellated cell of S. solfataricus P2. Bar, 200 nm.

Transcriptional regulation of the flaB gene.

We aimed to find conditions under which the flagellin gene is highly induced. Northern blot analysis was performed on RNA isolated from S. solfataricus P2 cells (obtained from DSMZ, Braunschweig, Germany) grown under different conditions, using part of the flaB gene as a probe. Cells were grown aerobically at 80°C and pH 3 in Brock's basal salt medium (minimal medium [MM]) (11). This medium was supplemented with either 0.2% tryptone (Difco), 0.1% yeast extract, and 0.2% sucrose (rich medium), or 0.2% sugar (glucose or arabinose). For analysis of flaB gene transcript levels, 5 μg of isolated total RNA (3) was separated on a denaturing 1.1% agarose gel and subsequently capillary blotted onto Zeta-Probe membranes (Bio-Rad). Nonradioactive hybridization and detection were performed as described previously (24). First, early and late growth phases of cells grown in rich medium were compared. While little flaB transcript was detected in samples from the mid-logarithmic growth phase, a strong induction in stationary cells was observed (Fig. 2B). We were unable to correlate the increased levels of flaB transcript to elevated amounts of flagella by electron microscopy. Log-phase-grown cells do contain flagella, while stationary-phase-grown cells could not be investigated by electron microscopy because they are highly prone to lysis (data not shown).

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FIG. 2.

Transcriptional regulation of flaB tested in S. solfataricus strain P2. (A) Northern analysis of flaB gene transcript after transfer of a cell culture from rich medium (TYS) to MM. RNA was isolated from cells before and 1 and 2 hours after the shift into either rich medium or MM. The flaB gene transcript was detected using a specific digoxigenin-labeled probe (top). The positions of the 16S and 23S ribosomal RNAs, determined by methylene blue staining of the blot membrane (bottom), are indicated. (B) Detection of flaB gene transcript levels at different growth stages by Northern analysis (top) and RT-PCR (middle). RNA was isolated from cells grown in rich medium to mid-logarithmic (L) or stationary (S) phase. Amounts of RNA identical to those used in the Northern blot analysis were separated on 1.5% agarose gels and stained with ethidium bromide (bottom). (C and D) Semiquantitative RT-PCR analysis of flaB expression levels in S. solfataricus cells either transferred from rich medium to MM (C) or grown on MM supplemented with a carbon source as indicated and harvested at an optical density of 0.6 (D). Samples for panel C were taken under conditions identical to those described for panel A. The upper panels show the PCR products, and the lower panels are a loading control for the RNA used in the RT-PCRs.

The induction might be due to a limitation of nutrient availability or a possible quorum-sensing mechanism (17). No homologs of bacterial quorum-sensing systems have been detected in archaea so far (10), although quorum sensing seems to exist as shown for the induction of an extracellular protease in Natronococcus occultus (30). To determine whether unfavorable nutritional conditions induce flaB expression, cells were shifted from rich medium to MM and samples were taken for Northern analysis. A cell culture grown in rich medium to mid-logarithmic phase was collected by centrifugation and resuspended in either rich medium or MM without the addition of a carbon source. Samples were taken before and 1 and 2 hours after the shift and analyzed as described above (Fig. 2A). No change in flaB transcript was observed when cells continued to grow on rich medium. However, a strong induction could be detected after 2 hours of incubation in MM. The up-regulation of flaB was confirmed by semiquantitative reverse transcriptase (RT) PCR performed on RNA isolated from cells grown under the conditions described above (Fig. 2B and C). RT-PCR was performed as described previously (3) with primers flaB1 (5′-AGACAGCGTCAACAGACTA-3′) and flaB2 (5′-ACCTGCACTTGTCTGCTGAT-3′). The absence of DNA contamination in all RNA preparations was confirmed by performing PCRs without prior reverse transcription (not shown). In addition, increased amounts of flaB transcript were detected in cells grown on sugars (glucose or arabinose) compared to that in cells grown with peptides (tryptone) or on rich medium (Fig. 2D). As peptide carbon sources are preferred over sugar MM conditions by S. solfataricus (26), the flagellin gene seems to be preferentially expressed in cells that are exposed to unfavorable nutritional conditions.

Construction and characterization of an flaJ disruption mutant.

To determine whether the flaJ accessory gene is involved in flagellar biogenesis and motility in S. solfataricus, a disruption mutant was constructed in strain PBL2025. S. solfataricus PBL2025 contains a large chromosomal deletion which also includes lacS, the gene coding for β-glycosidase, and accordingly, these cells are not able to grow on lactose as the sole carbon source (33). The flaJ gene was targeted using a suicide plasmid (pET2275) carrying the flaJ coding region interrupted at base 685 by the lacS gene as a selectable marker (Fig. 3A). The lacS gene with its own promoter and terminator region was cloned by PCR amplification (using primers with the sequences 5′-CCCCCCATGGCTCCTCTTATTATTAGAATTGTACGC-3′ and 5′-CCCCCGGATCCCTAGTGTTGCAAGGCAG-3′; NcoI and BamHI restriction sites are underlined, respectively), with S. solfataricus P2 genomic DNA as a template. The PCR product was ligated into NcoI- and BamHI-cut pET401 cloning vector (K. H. M. van Wely, unpublished data), yielding pET2268, and the endogenous EcoRV site of lacS was subsequently removed by site-directed mutagenesis (43). The N-terminal (673 bp) and C-terminal (728 bp) coding regions of flaJ were amplified by PCR using primers with the sequences 5′-CCCCGGTACCGCAAAAAATGAAGTAGACTCC-3′ and 5′-CCCCCCATGGCGATTCCTAATAGAACTATACCAG-3′ (KpnI and NcoI restriction sites are underlined, respectively) and 5′-CCCCGGATCCAGGTATAGATTGTATGAAAAT-3′ and 5′-CCCCCGCGGCCGCGTAATAAACCCGATGGTGATGC-3′ (BamHI and NotI restriction sites are underlined, respectively), respectively, with S. solfataricus P2 genomic DNA as a template. The cut PCR products were inserted into pET2268 by using the appropriate restriction enzymes, resulting in pET2275. After electroporation of S. solfataricus PBL2025 with pET2275, a strain carrying the integrant was selected on liquid MM supplemented with lactose and purified as described before (43). The integrity of the mutant was confirmed by PCR amplification of part of the flaJ gene region (Fig. 3A and B). As expected, using DNA from the wild-type strain as a template and primers flaJ1 (5′-GCTCCCTTACCTTTCTAGTAGC-3′) and flaJ2 (5′-GCCTTACGTCTCCTAGATCC-3′), a 428-base-pair fragment was amplified (Fig. 3B). A PCR performed on DNA isolated from the flaJ::lacS strain with the same primers resulted in a larger fragment of 2.4 kb corresponding to the flaJ gene carrying the lacS insert (Fig. 3B). Also, the two regions overlapping the flaJ flanks and the selection marker were amplified by PCR and analyzed by direct sequencing of the PCR products. The resulting flaJ::lacS mutant strain and the wild-type strain were characterized for motility and the presence of flagella. Cells grown in liquid glucose medium were negatively stained with 2% uranyl acetate and examined by transmission electron microscopy (Fig. 3C and D). Cells of the wild-type strain appeared to be peritrichous, without any bundling of filaments (Fig. 3C). Flagella were usually short with a slight wave-like curvature. Longer filaments of up to several micrometers in length were also observed. On the other hand, cells of the flaJ strain completely lacked flagella on the cell surface (Fig. 3D). To establish whether flagella conferred motility in S. solfataricus, a swarming assay on semisolid plates was established. To prepare semisolid medium, 0.2% Gelrite (Serva, Heidelberg, Germany), dissolved by stirring in boiling demineralized water, was added to an equal volume of two-times-concentrated MM containing 0.02% glucose and 20 mM magnesium chloride and 6 mM calcium chloride as solidifying agents. The pH of the medium was adjusted to 3. To inoculate the plates, cells grown on MM supplemented with 0.1% glucose were pelleted (10 min, 3,500 × g, 21°C) and resuspended in 0.1 volume of culture supernatant. Next, a 10-μl droplet of this suspension, corresponding to approximately 10⁷ cells, was applied to the center of the plate and allowed to absorb into the medium. Plates were incubated for 5 to 6 days in a sealed humid chamber at 80°C. Strain PBL2025 formed a dense circular spot of cells, corresponding to the area of inoculation, and a lighter halo around this region, consistent with a swarming phenotype (Fig. 3E). In contrast, no halo formation was observed with the flaJ cells (Fig. 3F). Therefore, the flaJ gene product is required for the biogenesis of flagella on S. solfataricus cells as well as for swarming motility. FlaJ is the only polytopic membrane protein in the fla operon, and based on its similarity to bacterial GspF proteins, it is tempting to speculate that it constitutes the platform on which the flagellum is assembled in the cytoplasmic membrane by the ATPase FlaI (3).

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FIG. 3.

Construction of an flaJ disruption mutant and characterization of wild-type versus mutant strains. (A) Schematic representation of the genomic environment of the flaJ gene and the suicide plasmid used to target the gene. Arrowheads indicate primers flaJ1 and flaJ2. The recognition sites of selected restriction enzymes on the genomic fragment are HindIII, EcoRI, and ScaI. (B) Results of a PCR on genomic DNA isolated from strain PBL2025-flaJ::lacS or strain PBL2025 (wild type [wt]). (C and D) Transmission electron microscopy images of negatively stained cells. Bar, 200 nm. (E and F) Motility was assayed on semisolid Gelrite plates supplemented with MM plus 0.05% glucose.

Structural features of isolated flagella.

Sulfolobus species carry only one copy of the flagellin gene (4, 34) and do not show reversed swimming or tumbling (20, 25). Therefore, we argued that the structure of flagella from this organism should be homogenous, in contrast to polymorphic flagella from H. salinarum that are built up of multiple subunits (40). To obtain structural information about the flagellar filament, a series of electron micrographs was collected for negatively stained specimens isolated from culture supernatants. A crude flagellar preparation was isolated from culture supernatants of S. solfataricus PBL2025 grown in MM supplemented with 0.1% glucose until stationary phase. Cells were removed by centrifugation (30 min, 3,000 × g, 4°C). The supernatant was recentrifuged, and the resulting culture supernatant was pelleted by ultracentrifugation (20 min, 26,000 × g, 4°C). This pellet contained numerous flagella as well as cellular debris and was used for initial electron microscopy observations. The centrifugation of the supernatant (45 min, 220,000 × g, 4°C) resulted in a pellet which consisted mainly of flagella. This material was used for detailed analysis of the flagellar ultrastructure. Flagellar filament suspensions were negatively stained with 2% uranyl acetate on glow-discharged carbon-coated copper grids. Flagella were observed on a Philips CM120 electron microscope, operating at 120 kV with a LaB6 filament. Images were recorded with a charge-coupled-device camera at ×80,000 magnification with a pixel size of 3.75 Å at the specimen level with GRACE software (29). Long filaments of up to several micrometers extending out of the grid plane were frequently observed (Fig. 4A). Segments of filaments which were rather straight were extracted from micrographs for image analysis (Fig. 4B). A single-particle approach (performed with the Groningen Image Processing software package) was used for processing (18). Slightly overlapping segments were extracted from the micrographs. To correct for the significant in-plane curvature, we first rotationally aligned all segments. The best 60% of the rotationally oriented segments, as judged by using the correlation coefficient as the quality criterion, were then further processed by full alignment procedures. The aligned projections were treated with multivariate statistical analysis in combination with hierarchical classification (41) before final averaging. The final average projection map of 120 segments clearly shows that the filament has a helical packing (Fig. 4D). The filament has a diameter of 145 Å and a pitch of 54 Å (Fig. 4D). Thus, the S. solfataricus flagellum is thicker than that of H. salinarum (approximately 100 Å) (14), probably due to the higher molecular weight of the S. solfataricus flagellin. The filament has an apparent three-stranded helical arrangement, as indicated in the scheme in Fig. 4E. The resolution of the two-dimensional map is about 18 Å. Surface metal shadowing was performed to determine the handedness of the expected helical packing of the filaments (Fig. 4C). The contribution of the helices in one direction, from the lower left to the upper right, was stronger than in the upper-left to lower-right direction (Fig. 4C). This indicated that the filaments are composed of right-handed helices, as is the case for the flagellum of H. salinarum (2, 14).

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FIG. 4.

Electron microscopy analysis of the S. solfataricus flagellum. (A) Example of a long flagellar filament showing a typical wave-like pattern. (B) A typical segment of a flagellum, used for image processing; the helical motif of the filament is readily recognizable. (C) The projection average of 33 aligned metal-shadowed segments indicates that the filament is right-handed. (D) Two-dimensional projection average of 120 aligned segments. (E) Scheme for the helical packing projected on the averaged image of frame (D). The three-start left-handed helical packing is indicated in color. Bars, 1 μm (A) and 10 nm (D and E).

In this study, we have investigated flagellation in S. solfataricus and established a basis for future investigations of cell surface-exposed structures. Because flagella are abundant on Sulfolobus cells, the availability of a nonflagellated mutant will significantly ease the investigation of other types of membrane-bound organelles, including pili (42) and the bindosome, a putative assembly of ABC transporter binding proteins involved in the efficient uptake of various sugars (3, 5).