Article Figures & Data
Figures
- FIG. 1.
Amino acid comparison between various Corynebacterium HmuO proteins and the human heme oxygenase HO-1. The numbers separated by a slash represent percent identity/similarity. Cd-NCTC, C. diphtheriae genome strain NCTC 13129 (8); Cd-C7, C7(−) strain; Cu, C. ulcerans 712; Cg, Corynebacterium glutamicum; Cjk, Corynebacterium jeikeium.
- FIG. 2.
A. Alignment of the nucleotide sequences in the hmuO promoter region between C. ulcerans (CU) and C. diphtheriae C7 (CD). DtxR binding sites are underlined. rbs, putative ribosome binding site; ATG, start codon for hmuO. The arrow indicates the start of transcription in C. diphtheriae (19). B. Alignment of the 19-bp DtxR binding sites from CU712 and C. diphtheriae C7 with the DtxR consensus binding sequence. Residues represented by bold characters indicate the most highly conserved nucleotides.
Tables
- TABLE 1.
Growth stimulation of Corynebacterium strains by iron and hemoglobin
Strain/plasmid Growth (OD600) with various iron sourcesa +Feb −Fec −Fe with hemoglobin (μg/ml) Hbf (50) Hb (25) Hb (10) Hb (5) CU712 wild type 4.5 <0.1 4.8 2.8 0.5 0.3 CU712 hmuOΔ 4.0 <0.1 0.14 0.10 ndd nd CU712 hmuOΔ/pCUhmuO 3.8 <0.1 4.2 2.5 nd nd CU712 hmuOΔ/p37hmuO 4.2 <0.1 4.1 2.2 nd nd CU712 hmuOΔ/p1841 3.8 <0.1 0.13 0.08 nd nd C7(−) wild type 2.1 <0.1 4.3 2.7 0.7 0.4 C7 hmuOΔ 2.2 <0.1 1.1 0.7 nd nd C7 hmuOΔ/pCM2.6 (vector) 5.7e <0.1 1.9 0.9 nd nd C7 hmuOΔ/pCD293 (hmuO+) 5.9 <0.1 5.5 2.6 nd nd C7 hmuOΔ-1841Δ 2.0 <0.1 0.9 0.5 nd nd 1737 wild type 6.4 <0.1 5.8 3.5 nd nd 1737 hmuOΔ 6.8 <0.1 6.6 3.3 nd nd ↵ a OD600 was determined for each culture after growth for 20 to 22 h in mPGT medium. Values shown are results from a representative experiment. All experiments were repeated at least three times, and the results of each assay varied by less than 20% from the mean.
↵ b +Fe medium is mPGT with 1 μM FeSO4.
↵ c −Fe medium is mPGT with 3.6 μg/ml EDDA.
↵ d nd, not done.
↵ e C7 strains carrying pCM2.6 plasmids grow to higher densities than strains without plasmids.
↵ f Hb, hemoglobin.
- TABLE 2.
hmuO promoter activity in Corynebacterium strains
Strain/plasmide LacZ activitya in: +Feb −Fec −Fe/Hbd C7 wt/pCP01 (C7/hmuO-lacZ) <0.5 3.0 (0.7) 112.0 (3.0) CU712 wt/pPO712 (CU/hmuO-lacZ) 46.7 (2.3) 129.7 (11.1) 256.6 (20.0) CU712 hmuOΔ/pPO712 121.3 (15.5) 196.0 (38.0) 922.0 (120.0) C7 wt/pPO712 10.9 (3.0) 15.5 (3.4) 155.3 (24.5) C7chrASΔ-hrrASΔ/ pPO712 1.7 (0.3) 2.6 (0.1) 2.5 (0.3)
