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ENZYMES AND PROTEINS

Zinc-Independent Folate Biosynthesis: Genetic, Biochemical, and Structural Investigations Reveal New Metal Dependence for GTP Cyclohydrolase IB

Banumathi Sankaran, Shilah A. Bonnett, Kinjal Shah, Scott Gabriel, Robert Reddy, Paul Schimmel, Dmitry A. Rodionov, Valérie de Crécy-Lagard, John D. Helmann, Dirk Iwata-Reuyl, Manal A. Swairjo
Banumathi Sankaran
1The Berkeley Center for Structural Biology, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, California 94720
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Shilah A. Bonnett
2Department of Chemistry, Portland State University, P.O. Box 751, Portland, Oregon 97207
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Kinjal Shah
3Department of Basic Medical Sciences, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, 309 E. 2nd Street, Pomona, California 91766-1854
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Scott Gabriel
4Department of Microbiology, Cornell University, 327 Wing Hall, Ithaca, New York 14853-8101
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Robert Reddy
5Departments of Chemistry and Molecular Biology, The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N. Torrey Pines Rd., BCC-379, La Jolla, California 92037
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Paul Schimmel
5Departments of Chemistry and Molecular Biology, The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N. Torrey Pines Rd., BCC-379, La Jolla, California 92037
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Dmitry A. Rodionov
6Burnham Institute for Medical Research, 10901 N. Torrey Pines Rd., La Jolla, California 92037
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Valérie de Crécy-Lagard
7Department of Microbiology and Cell Science, University of Florida, P.O. Box 110700, Gainesville, Florida 32611-0700
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John D. Helmann
4Department of Microbiology, Cornell University, 327 Wing Hall, Ithaca, New York 14853-8101
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Dirk Iwata-Reuyl
2Department of Chemistry, Portland State University, P.O. Box 751, Portland, Oregon 97207
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  • For correspondence: iwatareuyld@pdx.edu mswairjo@burnham.org
Manal A. Swairjo
3Department of Basic Medical Sciences, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, 309 E. 2nd Street, Pomona, California 91766-1854
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  • For correspondence: iwatareuyld@pdx.edu mswairjo@burnham.org
DOI: 10.1128/JB.00287-09
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Article Information

DOI 
https://doi.org/10.1128/JB.00287-09
PubMed 
19767425

Published By 
American Society for Microbiology Journals
History 
  • Received March 4, 2009
  • Accepted September 14, 2009
  • Published online October 27, 2009.

Copyright & Usage 
Copyright © 2009 American Society for Microbiology

Article Versions

  • Accepted Manuscript version (September 18, 2009).
  • You are viewing the most recent version of this article.

Author Information

  1. Banumathi Sankaran1,
  2. Shilah A. Bonnett2,
  3. Kinjal Shah3¶,
  4. Scott Gabriel4,
  5. Robert Reddy5§,
  6. Paul Schimmel5,
  7. Dmitry A. Rodionov6,
  8. Valérie de Crécy-Lagard7,
  9. John D. Helmann4,
  10. Dirk Iwata-Reuyl2* and
  11. Manal A. Swairjo3*
  1. 1The Berkeley Center for Structural Biology, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, California 94720
  2. 2Department of Chemistry, Portland State University, P.O. Box 751, Portland, Oregon 97207
  3. 3Department of Basic Medical Sciences, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, 309 E. 2nd Street, Pomona, California 91766-1854
  4. 4Department of Microbiology, Cornell University, 327 Wing Hall, Ithaca, New York 14853-8101
  5. 5Departments of Chemistry and Molecular Biology, The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N. Torrey Pines Rd., BCC-379, La Jolla, California 92037
  6. 6Burnham Institute for Medical Research, 10901 N. Torrey Pines Rd., La Jolla, California 92037
  7. 7Department of Microbiology and Cell Science, University of Florida, P.O. Box 110700, Gainesville, Florida 32611-0700
  • ↵* Corresponding author. Mailing address for D. Iwata-Reuyl: Department of Chemistry, Portland State University, P.O. Box 751, Portland, OR 97207. Phone: (503) 725-5737. Fax: (503) 725-9525. E-mail: iwatareuyld{at}pdx.edu . Present address for M. A. Swairjo: Burnham Institute for Medical Research, 10901 N. Torrey Pines Rd., La Jolla, CA 92037. Phone: (619) 206-3864. Fax: (858) 795-5230. E-mail: mswairjo{at}burnham.org
  • ↵¶ Present address: Long Beach Public Health Lab, 2525 Grand Ave., Room 260, Long Beach, CA 90815.; § Present address: Human BioMolecular Research Institute, 5310 Eastgate Mall, San Diego, CA 92121-2804.

  • ‡ Supplemental material for this article may be found at http://jb.asm.org/ .

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Zinc-Independent Folate Biosynthesis: Genetic, Biochemical, and Structural Investigations Reveal New Metal Dependence for GTP Cyclohydrolase IB
Banumathi Sankaran, Shilah A. Bonnett, Kinjal Shah, Scott Gabriel, Robert Reddy, Paul Schimmel, Dmitry A. Rodionov, Valérie de Crécy-Lagard, John D. Helmann, Dirk Iwata-Reuyl, Manal A. Swairjo
Journal of Bacteriology Oct 2009, 191 (22) 6936-6949; DOI: 10.1128/JB.00287-09

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Zinc-Independent Folate Biosynthesis: Genetic, Biochemical, and Structural Investigations Reveal New Metal Dependence for GTP Cyclohydrolase IB
Banumathi Sankaran, Shilah A. Bonnett, Kinjal Shah, Scott Gabriel, Robert Reddy, Paul Schimmel, Dmitry A. Rodionov, Valérie de Crécy-Lagard, John D. Helmann, Dirk Iwata-Reuyl, Manal A. Swairjo
Journal of Bacteriology Oct 2009, 191 (22) 6936-6949; DOI: 10.1128/JB.00287-09
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KEYWORDS

Bacterial Proteins
Folic Acid
GTP Cyclohydrolase
zinc

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