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GENE REGULATION

Down-Regulation of the Escherichia coli K-12 nrf Promoter by Binding of the NsrR Nitric Oxide-Sensing Transcription Repressor to an Upstream Site

Douglas F. Browning, David J. Lee, Stephen Spiro, Stephen J. W. Busby
Douglas F. Browning
1School of Biosciences, University of Birmingham, Birmingham B15 2TT, United Kingdom
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  • For correspondence: D.F.Browning@bham.ac.uk
David J. Lee
1School of Biosciences, University of Birmingham, Birmingham B15 2TT, United Kingdom
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Stephen Spiro
2Department of Molecular and Cell Biology, The University of Texas at Dallas, Richardson, Texas 75080
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Stephen J. W. Busby
1School of Biosciences, University of Birmingham, Birmingham B15 2TT, United Kingdom
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DOI: 10.1128/JB.00218-10
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Article Information

DOI 
https://doi.org/10.1128/JB.00218-10
PubMed 
20472787

Published By 
American Society for Microbiology Journals
History 
  • Received March 1, 2010
  • Accepted May 9, 2010
  • Published online June 25, 2010.

Copyright & Usage 
Copyright © 2010 American Society for Microbiology

Article Versions

  • Accepted Manuscript version (May 14, 2010).
  • You are viewing the most recent version of this article.

Author Information

  1. Douglas F. Browning1*,
  2. David J. Lee1,
  3. Stephen Spiro2 and
  4. Stephen J. W. Busby1
  1. 1School of Biosciences, University of Birmingham, Birmingham B15 2TT, United Kingdom
  2. 2Department of Molecular and Cell Biology, The University of Texas at Dallas, Richardson, Texas 75080
  • ↵* Corresponding author. Mailing address: School of Biosciences, University of Birmingham, Birmingham B15 2TT, United Kingdom. Phone: 44-121-414-5434. Fax: 44-121-414-5925. E-mail: D.F.Browning{at}bham.ac.uk

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Down-Regulation of the Escherichia coli K-12 nrf Promoter by Binding of the NsrR Nitric Oxide-Sensing Transcription Repressor to an Upstream Site
Douglas F. Browning, David J. Lee, Stephen Spiro, Stephen J. W. Busby
Journal of Bacteriology Jun 2010, 192 (14) 3824-3828; DOI: 10.1128/JB.00218-10

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Down-Regulation of the Escherichia coli K-12 nrf Promoter by Binding of the NsrR Nitric Oxide-Sensing Transcription Repressor to an Upstream Site
Douglas F. Browning, David J. Lee, Stephen Spiro, Stephen J. W. Busby
Journal of Bacteriology Jun 2010, 192 (14) 3824-3828; DOI: 10.1128/JB.00218-10
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  • Top
  • Article
    • ABSTRACT
    • Deletion analysis of pnrf.
    • Mutational analysis of the NsrR binding site.
    • Gel retardation assays.
    • NsrR and IHF corepress at pnrf.
    • DNA sampling of the nrf promoter.
    • NsrR does not regulate the Salmonella nrf promoter.
    • Discussion.
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
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KEYWORDS

DNA-binding proteins
Escherichia coli K12
Escherichia coli Proteins
nitric oxide
Promoter Regions, Genetic
transcription factors

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