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Articles

Lactate Utilization Is Regulated by the FadR-Type Regulator LldR in Pseudomonas aeruginosa

Chao Gao, Chunhui Hu, Zhaojuan Zheng, Cuiqing Ma, Tianyi Jiang, Peipei Dou, Wen Zhang, Bin Che, Yujiao Wang, Min Lv, Ping Xu
Chao Gao
aState Key Laboratory of Microbial Technology, Shandong University, Jinan, People's Republic of China
bState Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, People's Republic of China
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Chunhui Hu
aState Key Laboratory of Microbial Technology, Shandong University, Jinan, People's Republic of China
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Zhaojuan Zheng
aState Key Laboratory of Microbial Technology, Shandong University, Jinan, People's Republic of China
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Cuiqing Ma
aState Key Laboratory of Microbial Technology, Shandong University, Jinan, People's Republic of China
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Tianyi Jiang
aState Key Laboratory of Microbial Technology, Shandong University, Jinan, People's Republic of China
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Peipei Dou
aState Key Laboratory of Microbial Technology, Shandong University, Jinan, People's Republic of China
bState Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, People's Republic of China
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Wen Zhang
aState Key Laboratory of Microbial Technology, Shandong University, Jinan, People's Republic of China
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Bin Che
aState Key Laboratory of Microbial Technology, Shandong University, Jinan, People's Republic of China
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Yujiao Wang
aState Key Laboratory of Microbial Technology, Shandong University, Jinan, People's Republic of China
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Min Lv
aState Key Laboratory of Microbial Technology, Shandong University, Jinan, People's Republic of China
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Ping Xu
aState Key Laboratory of Microbial Technology, Shandong University, Jinan, People's Republic of China
bState Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, People's Republic of China
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DOI: 10.1128/JB.06579-11
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ABSTRACT

NAD-independent l-lactate dehydrogenase (l-iLDH) and NAD-independent d-lactate dehydrogenase (d-iLDH) activities are induced coordinately by either enantiomer of lactate in Pseudomonas strains. Inspection of the genomic sequences of different Pseudomonas strains revealed that the lldPDE operon comprises 3 genes, lldP (encoding a lactate permease), lldD (encoding an l-iLDH), and lldE (encoding a d-iLDH). Cotranscription of lldP, lldD, and lldE in Pseudomonas aeruginosa strain XMG starts with the base, C, that is located 138 bp upstream of the lldP ATG start codon. The lldPDE operon is located adjacent to lldR (encoding an FadR-type regulator, LldR). The gel mobility shift assays revealed that the purified His-tagged LldR binds to the upstream region of lldP. An XMG mutant strain that constitutively expresses d-iLDH and l-iLDH was found to contain a mutation in lldR that leads to an Ile23-to-serine substitution in the LldR protein. The mutated protein, LldRM, lost its DNA-binding activity. A motif with a hyphenated dyad symmetry (TGGTCTTACCA) was identified as essential for the binding of LldR to the upstream region of lldP by using site-directed mutagenesis. l-Lactate and d-lactate interfered with the DNA-binding activity of LldR. Thus, l-iLDH and d-iLDH were expressed when the operon was induced in the presence of l-lactate or d-lactate.

FOOTNOTES

    • Received 28 November 2011.
    • Accepted 1 March 2012.
    • Accepted manuscript posted online 9 March 2012.
  • Supplemental material for this article may be found at http://dx.doi.org/10.1128/JB.06579-11.

  • Copyright © 2012, American Society for Microbiology. All Rights Reserved.
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Lactate Utilization Is Regulated by the FadR-Type Regulator LldR in Pseudomonas aeruginosa
Chao Gao, Chunhui Hu, Zhaojuan Zheng, Cuiqing Ma, Tianyi Jiang, Peipei Dou, Wen Zhang, Bin Che, Yujiao Wang, Min Lv, Ping Xu
Journal of Bacteriology Apr 2012, 194 (10) 2687-2692; DOI: 10.1128/JB.06579-11

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Lactate Utilization Is Regulated by the FadR-Type Regulator LldR in Pseudomonas aeruginosa
Chao Gao, Chunhui Hu, Zhaojuan Zheng, Cuiqing Ma, Tianyi Jiang, Peipei Dou, Wen Zhang, Bin Che, Yujiao Wang, Min Lv, Ping Xu
Journal of Bacteriology Apr 2012, 194 (10) 2687-2692; DOI: 10.1128/JB.06579-11
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