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GENOME ANNOUNCEMENTS

Whole-Genome Sequence of the Hypervirulent Clinical Strain Mycobacterium intracellulare M.i.198

Yoshitaka Tateishi, Seigo Kitada, Keisuke Miki, Ryoji Maekura, Yoshitoshi Ogura, Yuriko Ozeki, Yukiko Nishiuchi, Mamiko Niki, Tetsuya Hayashi, Kazuto Hirata, Kazuo Kobayashi, Sohkichi Matsumoto
Yoshitaka Tateishi
aDepartment of Bacteriology, Graduate School of Medicine, Osaka City University, Osaka, Japan
bNational Hospital Organization, Toneyama Hospital, Osaka, Japan
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Seigo Kitada
bNational Hospital Organization, Toneyama Hospital, Osaka, Japan
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Keisuke Miki
bNational Hospital Organization, Toneyama Hospital, Osaka, Japan
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Ryoji Maekura
bNational Hospital Organization, Toneyama Hospital, Osaka, Japan
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Yoshitoshi Ogura
cDepartment of Life Science, Frontier Science Research Center, University of Miyazaki, Miyazaki, Japan
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Yuriko Ozeki
aDepartment of Bacteriology, Graduate School of Medicine, Osaka City University, Osaka, Japan
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Yukiko Nishiuchi
aDepartment of Bacteriology, Graduate School of Medicine, Osaka City University, Osaka, Japan
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Mamiko Niki
aDepartment of Bacteriology, Graduate School of Medicine, Osaka City University, Osaka, Japan
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Tetsuya Hayashi
cDepartment of Life Science, Frontier Science Research Center, University of Miyazaki, Miyazaki, Japan
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Kazuto Hirata
dDepartment of Respiratory Medicine, Graduate School of Medicine, Osaka City University, Osaka, Japan
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Kazuo Kobayashi
eDepartment of Immunology, National Institute of Infectious Diseases, Tokyo, Japan
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Sohkichi Matsumoto
aDepartment of Bacteriology, Graduate School of Medicine, Osaka City University, Osaka, Japan
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DOI: 10.1128/JB.01439-12
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ABSTRACT

We report herein the draft genome sequence of Mycobacterium intracellulare clinical strain M.i.198, which consistently exhibits hypervirulence in human patients, human macrophages in vitro, and immunocompetent mice.

GENOME ANNOUNCEMENT

Nontuberculous mycobacteria are of great clinical importance with respect to the recent increasing prevalence of nontuberculous mycobacterioses, such as Mycobacterium avium complex (MAC) disease, which is difficult to treat without specific antibiotics (1). However, the bacteriological etiology of nontuberculous mycobacteria remains to be elucidated. We identified a hypervirulent Mycobacterium intracellulare strain (M.i.198) from an immunocompetent patient with pulmonary MAC disease (5). To help understand the genetic background of the virulence, we performed whole-genome sequencing of strain M.i.198.

We sequenced M. intracellulare M.i.198 genomic DNA on a Roche 454 FLX Titanium sequencer and assembled the reads using the software program Newbler v2.3. A total of 924,616 reads was generated, with an average read length of 246 bp, yielding a total sequence of 227,524,944 bp.

The assembled sequences contained 149 contigs, and the length of all contigs combined was 5,406,664 bp, with a G+C ratio of 68.0%. The average coverage depth was 42.1×, the N50 contig size was 90,025 bp, the average contig was 40,008 bp long, and the longest contig was 453,296 bp. The final assembly comprised 122 contigs in five scaffolds (99.7% of contig bases). We constructed an optical map (OpGen) of strain M.i.198 with the NheI restriction enzyme, yielding 311 ordered restriction fragments (average fragment size, 16 kb). The genome size was estimated to be approximately 5.22 Mb; this was obtained from the sum of all restriction fragments. The five scaffolds were placed on the map, confirming a single circular chromosome without plasmids.

Genome annotation using the NCBI Prokaryotic Genomes Automatic Annotation Pipeline (http://www.ncbi.nlm.nih.gov/genomes/static/Pipeline.html) identified 5,146 coding sequences (CDSs). Strain M.i.198 has three rRNAs (in a single rRNA operon) and 48 tRNA genes.

We compared the sequence of M.i.198 with those of three other recently sequenced M. intracellulare strains: ATCC 13950 (GenBank accession no. CP003322.1), MOTT-02 (GenBank accession no. CP003323.1), and MOTT-64 (GenBank accession no. CP003324.1)—the latter two were clinical isolates from Korean patients (2, 3, 4). The reciprocal best-hit BLAST approach revealed that strain M.i.198 shares 90.0%, 92.7%, and 88.7% of its CDSs with M. intracellulare ATCC 13950, MOTT-02, and MOTT-64, respectively. Of particular interest is a 51-kb region of difference, 55 CDSs in length (G+C ratio, 61.0%), which consists of prophages in M.i.198. The genome sequence of strain M.i.198 will provide an invaluable resource for understanding the microbiological aspects of human-pathogen interactions, especially the pathogenesis of human MAC disease.

Nucleotide sequence accession numbers.The whole-genome sequence of M.i.198 has been deposited in DDBJ/EMBL/GenBank under the accession numbers BAGQ01000001 to BAGQ01000149.

ACKNOWLEDGMENTS

This work was funded by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan, the Ministry of Health, Labor, and Welfare (Research on Emerging and Reemerging Infectious Diseases, Health Sciences Research Grants) of Japan, the Japan Health Sciences Foundation, and the United States-Japan Cooperative Medical Science Program against Tuberculosis and Leprosy.

FOOTNOTES

    • Received 10 August 2012.
    • Accepted 10 September 2012.
  • Copyright © 2012, American Society for Microbiology. All Rights Reserved.

REFERENCES

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    1. Griffith DE,
    2. et al
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    1. Kim BJ,
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Whole-Genome Sequence of the Hypervirulent Clinical Strain Mycobacterium intracellulare M.i.198
Yoshitaka Tateishi, Seigo Kitada, Keisuke Miki, Ryoji Maekura, Yoshitoshi Ogura, Yuriko Ozeki, Yukiko Nishiuchi, Mamiko Niki, Tetsuya Hayashi, Kazuto Hirata, Kazuo Kobayashi, Sohkichi Matsumoto
Journal of Bacteriology Oct 2012, 194 (22) 6336; DOI: 10.1128/JB.01439-12

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Whole-Genome Sequence of the Hypervirulent Clinical Strain Mycobacterium intracellulare M.i.198
Yoshitaka Tateishi, Seigo Kitada, Keisuke Miki, Ryoji Maekura, Yoshitoshi Ogura, Yuriko Ozeki, Yukiko Nishiuchi, Mamiko Niki, Tetsuya Hayashi, Kazuto Hirata, Kazuo Kobayashi, Sohkichi Matsumoto
Journal of Bacteriology Oct 2012, 194 (22) 6336; DOI: 10.1128/JB.01439-12
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