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Commentary

Iron-Mediated Control of Pseudomonas aeruginosa-Staphylococcus aureus Interactions in the Cystic Fibrosis Lung

Patricia M. Barnabie, Marvin Whiteley
V. J. DiRita, Editor
Patricia M. Barnabie
Department of Molecular Biosciences, Institute of Cellular and Molecular Biology, Center for Infectious Disease, The University of Texas at Austin, Austin, Texas, USA
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Marvin Whiteley
Department of Molecular Biosciences, Institute of Cellular and Molecular Biology, Center for Infectious Disease, The University of Texas at Austin, Austin, Texas, USA
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V. J. DiRita
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DOI: 10.1128/JB.00303-15
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ABSTRACT

Communication is an important factor for bacterial survival, growth, and persistence. Much work has examined both inter- and intraspecies interactions and their effects on virulence. Now, researchers have begun to explore the ways in which host-modulated factors can impact bacterial interactions and subsequently affect patient outcomes. In this issue, two papers discuss how the host environment alters interactions between the pathogens Pseudomonas aeruginosa and Staphylococcus aureus, largely in the context of cystic fibrosis.

The views expressed in this Commentary do not necessarily reflect the views of the journal or of ASM.

MICROSCOPIC SOCIETY

Just as macroscopic creatures must communicate in order to survive and thrive in the macroscopic world, so too do microorganisms “talk” with each other at the microscopic level. Instead of audio calls and visual cues, bacteria instead rely on the language of signaling molecules. As this method of cellular communication involves large numbers of cells coming to a “consensus” on subsequent group actions, researchers dubbed this behavior “quorum sensing.” Classical quorum sensing has been largely focused on autoinduction, wherein a single species produces a signaling molecule unable to induce gene expression until the population grows to a threshold concentration. This phenomenon was first characterized in bioluminescence produced by Aliivibrio (formerly Vibrio) fischeri and has since been identified in a variety of clinically relevant human pathogens. More recent cell-cell communication research has revealed that other kinds of molecules, including metabolites and nutrients present in the growth environment, serve as cues that impact bacterial interactions (1–3). Additionally, interactions between different species have been found to occur in polymicrobial infections where they often form synergistic, virulence-enhancing relationships (4).

MICRONUTRIENTS/MICROORGANISMS

As pathogenic bacteria prey upon their host as a living source of nourishment, it is not surprising that available nutrients in the host environment can have a major impact on microbial gene expression. It also follows, then, that such an important regulatory factor might also affect the ways in which bacteria interact within the host. Therefore, a key question is whether the in vitro systems primarily used to study microbial interactions provide relevant insights into the ways in which microbes interact in the host.

This issue of the Journal of Bacteriology features two papers that explore the impact of host-provided nutrients further in the context of iron's ability to modulate Pseudomonas aeruginosa/Staphylococcus aureus interactions in the cystic fibrosis (CF) lung. Iron is a micronutrient vital for bacterial growth but relatively scarce in most infection sites, acting as a limiting factor (5). In contrast, iron levels have been found to be high in CF sputum and correlate negatively with patient outcomes (6). Additionally, pathogen lung colonization in CF patients displays a conserved pattern of succession, with S. aureus predominating early on before being displaced by P. aeruginosa (7). Although it has been previously demonstrated that P. aeruginosa harvests iron via killing of S. aureus (8), the exact dynamics of this hostile takeover remain unclear. Here, Filkins et al. (7) put forward an interesting hypothesis regarding progressional acquisition, along with supporting in vitro experiments. Based on gene expression data from bacteria cocultured on CF bronchiolar epithelial cells, these researchers discovered that the presence of P. aeruginosa drives S. aureus toward fermentative metabolism. This feat is accomplished via sabotaging S. aureus's aerobic metabolism with a combination of factors, including the excretion of the potent staphylococcal respiratory chain inhibitor 2-heptyl-4-hydroxyquinoline N-oxide along with iron-scavenging siderophores. The shift proves detrimental to S. aureus growth rates and fitness, while these conditions are more than hospitable for P. aeruginosa, which preferentially feeds on the lactate that its victim produces (7). Relevant to this model, Nguyen et al. also demonstrate that free iron levels regulate P. aeruginosa's inhibition of S. aureus in coculture (9). This group had previously shown that P. aeruginosa's production of specific 2-alkyl-4(1H)-quinolones, antimicrobial compounds capable of killing S. aureus, is increased in low-iron environments (5). Here, they further demonstrate that this particular environmentally mediated form of bacterial cross-species inhibition occurs over a variety of P. aeruginosa strains, including those isolated from CF patients. Additionally, when P. aeruginosa is grown in monoculture, high iron levels are able to suppress production of the antimicrobials that target S. aureus. Moreover, when these two bacteria are grown together in coculture, iron's ability to suppress antimicrobial production is restored to CF isolates previously thought to be defective for this activity. These findings suggest that bacterial iron-regulated pathways become altered in polymicrobial infections and may contribute significantly to pathogenesis (9).

HOST-PATHOGEN-PATHOGEN INTERACTIONS

As antibiotic resistance rates skyrocket and the threat of a postantibiotic era looms, the importance of alternative treatment modalities in clinical microbiology is clear. This is especially relevant in the context of polymicrobial infections, which display inherent resistance to traditional antibiotics (4). One promising method may be the development of therapeutics that target specific bacterial virulence factors, which have been proposed to reduce morbidity and mortality without promoting antimicrobial resistance through direct fitness selection. However, in order for these targeted therapies to become a reality, much needs to be learned regarding the exact mechanisms leading to virulence and poor patient outcomes. The two papers presented here give some of the first evidence regarding how host micronutrients impact interactions of two prevalent CF pathogens and provide insights into the mechanism controlling P. aeruginosa-mediated succession in the CF lung. The control of such host-modulated bacterial interactions is thus a largely unexplored field that may one day prove a fertile ground for medical innovation.

FOOTNOTES

    • Accepted manuscript posted online 27 April 2015.
  • Address correspondence to Marvin Whiteley, mwhiteley{at}austin.utexas.edu.
  • Citation Barnabie PM, Whiteley M. 2015. Iron-mediated control of Pseudomonas aeruginosa-Staphylococcus aureus interactions in the cystic fibrosis lung. J Bacteriol 197:2250–2251. doi:10.1128/JB.00303-15.

REFERENCES

  1. 1.↵
    1. Fuqua C,
    2. Winans SC,
    3. Greenberg EP
    . 1996. Census and consensus in bacterial ecosystems: the LuxR-LuxI family of quorum-sensing transcriptional regulators. Annu Rev Microbiol 50:727–751. doi:10.1146/annurev.micro.50.1.727.
    OpenUrlCrossRefPubMedWeb of Science
  2. 2.↵
    1. Rutherford ST,
    2. Bassler BL
    . 2012. Bacterial quorum sensing: its role in virulence and possibilities for its control. Cold Spring Harb Perspect Med 2:a012427. doi:10.1101/cshperspect.a012427.
    OpenUrlAbstract/FREE Full Text
  3. 3.↵
    1. Urbanczyk H,
    2. Ast JC,
    3. Higgins MJ,
    4. Carson J,
    5. Dunlap PV
    . 2007. Reclassification of Vibrio fischeri, Vibrio logei, Vibrio salmonicida and Vibrio wodanis as Aliivibrio fischeri gen. nov., comb. nov., Aliivibrio logei comb. nov., Aliivibrio salmonicida comb. nov. and Aliivibrio wodanis comb. nov. Int J Syst Evol Microbiol 57:2823–2829. doi:10.1099/ijs.0.65081-0.
    OpenUrlCrossRefPubMedWeb of Science
  4. 4.↵
    1. Murray JL,
    2. Connell JL,
    3. Stacy A,
    4. Turner KH,
    5. Whiteley M
    . 2014. Mechanisms of synergy in polymicrobial infections. J Microbiol 52:188–199. doi:10.1007/s12275-014-4067-3.
    OpenUrlCrossRefPubMed
  5. 5.↵
    1. Nguyen AT,
    2. Oglesby-Sherrouse AG
    . 2015. Spoils of war: iron at the crux of clinical and ecological fitness of Pseudomonas aeruginosa. Biometals doi:10.1007/s10534-015-9848-6.
    OpenUrlCrossRef
  6. 6.↵
    1. Gifford AH,
    2. Miller SD,
    3. Jackson BP,
    4. Hampton TH,
    5. O'Toole GA,
    6. Stanton BA,
    7. Parker HW
    . 2011. Iron and CF-related anemia: expanding clinical and biochemical relationships. Pediatr Pulmonol 46:160–165. doi:10.1002/ppul.21335.
    OpenUrlCrossRefPubMed
  7. 7.↵
    1. Filkins LM,
    2. Graber JA,
    3. Olson DG,
    4. Dolben EL,
    5. Lynd LR,
    6. Bhuju S,
    7. O'Toole GA
    . 2015. Coculture of Staphylococcus aureus with Pseudomonas aeruginosa drives S. aureus towards fermentative metabolism and reduced viability in a cystic fibrosis model. J Bacteriol 197:2252–2264. doi:10.1128/JB.00059-15.
    OpenUrlAbstract/FREE Full Text
  8. 8.↵
    1. Mashburn LM,
    2. Jett AM,
    3. Akins DR,
    4. Whiteley M
    . 2005. Staphylococcus aureus serves as an iron source for Pseudomonas aeruginosa during in vivo coculture. J Bacteriol 187:554–566. doi:10.1128/JB.187.2.554-566.2005.
    OpenUrlAbstract/FREE Full Text
  9. 9.↵
    1. Nguyen AT,
    2. Jones JW,
    3. Ruge MA,
    4. Kane MA,
    5. Oglesby-Sherrouse AG
    . 2015. Iron depletion enhances production of antimicrobials by Pseudomonas aeruginosa. J Bacteriol 197:2265–2275. doi:10.1128/JB.00072-15.
    OpenUrlAbstract/FREE Full Text
  • Copyright © 2015, American Society for Microbiology. All Rights Reserved.
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Iron-Mediated Control of Pseudomonas aeruginosa-Staphylococcus aureus Interactions in the Cystic Fibrosis Lung
Patricia M. Barnabie, Marvin Whiteley
Journal of Bacteriology Jun 2015, 197 (14) 2250-2251; DOI: 10.1128/JB.00303-15

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Iron-Mediated Control of Pseudomonas aeruginosa-Staphylococcus aureus Interactions in the Cystic Fibrosis Lung
Patricia M. Barnabie, Marvin Whiteley
Journal of Bacteriology Jun 2015, 197 (14) 2250-2251; DOI: 10.1128/JB.00303-15
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