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Research Article

Genetic Control of Oxidative Mutagenesis in Sulfolobus acidocaldarius

Rupal Jain, Samuel Dhiman, Dennis W. Grogan
William W. Metcalf, Editor
Rupal Jain
aDepartment of Biological Sciences, University of Cincinnati, Cincinnati, Ohio, USA
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Samuel Dhiman
aDepartment of Biological Sciences, University of Cincinnati, Cincinnati, Ohio, USA
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Dennis W. Grogan
aDepartment of Biological Sciences, University of Cincinnati, Cincinnati, Ohio, USA
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William W. Metcalf
University of Illinois at Urbana-Champaign
Roles: Editor
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DOI: 10.1128/JB.00756-19
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ABSTRACT

To identify DNA oxidation defenses of hyperthermophilic archaea, we deleted genes encoding the putative 7,8-dihydro-8-oxoguanine (oxoG)-targeted N-glycosylase of Sulfolobus acidocaldarius (ogg; Saci_01367), the Y family DNA polymerase (dbh; Saci_0554), or both and measured the effects on cellular survival, replication accuracy, and oxoG bypass in vivo. Spontaneous G·C-to-T·A transversions were elevated in all Δogg and Δdbh constructs, and the Δogg Δdbh double mutant lost viability at a higher rate than isogenic wild-type (WT) and ogg strains. The distribution of G·C-to-T·A transversions within mutation detector genes suggested that reactivity of G toward oxidation and the effect on translation contribute heavily to the pattern of mutations that are recovered. An impact of the Ogg protein on the overall efficiency of bypassing oxoG in transforming DNA was evident only in the absence of Dbh, and Ogg status did not affect the accuracy of bypass. Dbh function, in contrast, dramatically influenced both the efficiency and accuracy of oxoG bypass. Thus, Ogg and Dbh were found to work independently to avoid mutagenesis by oxoG, and inactivating this simple but effective defense system by deleting both genes imposed a severe mutational burden on S. acidocaldarius cells.

IMPORTANCE Hyperthermophilic archaea are expected to have effective (and perhaps atypical) mechanisms to limit the genetic consequences of DNA damage, but few gene products have been demonstrated to have genome-preserving functions in vivo. This study confirmed by genetic criteria that the S. acidocaldarius Ogg protein avoids the characteristic mutagenesis of G oxidation. This enzyme and the bypass polymerase Dbh have similar impacts on genome stability but work independently and may comprise most of the DNA oxidation defense of S. acidocaldarius. The critical dependence of accurate oxoG bypass on the accessory DNA polymerase Dbh further argues that some form of polymerase exchange is important for accurate genome replication in Sulfolobus, and perhaps in related hyperthermophilic archaea.

FOOTNOTES

    • Received 11 December 2019.
    • Accepted 26 May 2020.
    • Accepted manuscript posted online 1 June 2020.
  • Supplemental material is available online only.

  • Copyright © 2020 American Society for Microbiology.

All Rights Reserved.

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Genetic Control of Oxidative Mutagenesis in Sulfolobus acidocaldarius
Rupal Jain, Samuel Dhiman, Dennis W. Grogan
Journal of Bacteriology Jul 2020, 202 (16) e00756-19; DOI: 10.1128/JB.00756-19

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Genetic Control of Oxidative Mutagenesis in Sulfolobus acidocaldarius
Rupal Jain, Samuel Dhiman, Dennis W. Grogan
Journal of Bacteriology Jul 2020, 202 (16) e00756-19; DOI: 10.1128/JB.00756-19
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KEYWORDS

7,8-dihydro-8-oxoguanine
Archaea
base excision repair
hyperthermophile
spontaneous mutations
translesion DNA polymerase

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