PT  - JOURNAL ARTICLE
AU  - Margolin, Y
AU  - Barak, R
AU  - Eisenbach, M
TI  - Arsenate arrests flagellar rotation in cytoplasm-free envelopes of bacteria.
AID  - 10.1128/jb.176.17.5547-5549.1994
DP  - 1994 Sep 01
TA  - Journal of Bacteriology
PG  - 5547--5549
VI  - 176
IP  - 17
4099  - http://jb.asm.org/content/176/17/5547.short
4100  - http://jb.asm.org/content/176/17/5547.full
SO  - J. Bacteriol.1994 Sep 01; 176
AB  - The effect of arsenate on flagellar rotation in cytoplasm-free flagellated envelopes of Escherichia coli and Salmonella typhimurium was investigated. Flagellar rotation ceased as soon as the envelopes were exposed to arsenate. Inclusion of phosphate intracellularly (but not extracellular) prevented the inhibition by arsenate. In a parallel experiment, the rotation was not affected by inclusion of an ATP trap (hexokinase and glucose) within the envelopes. It is concluded that arsenate affects the motor in a way other than reversible deenergization. This may be an irreversible damage to the cell or direct inhibition of the motor by arsenate. The latter possibility suggests that a process of phosphorylation or phosphate binding is involved in the motor function.