PT  - JOURNAL ARTICLE
AU  - Kilburn, Laurie
AU  - Poole, Keith
AU  - Meyer, Jean-Marie
AU  - Neshat, Shadi
TI  - Insertion Mutagenesis of the Ferric Pyoverdine Receptor FpvA of<em>Pseudomonas aeruginosa</em>: Identification of Permissive Sites  and a Region Important for Ligand Binding
DP  - 1998 Dec 15
TA  - Journal of Bacteriology
PG  - 6753--6756
VI  - 180
IP  - 24
4099  - http://jb.asm.org/content/180/24/6753.short
4100  - http://jb.asm.org/content/180/24/6753.full
SO  - J. Bacteriol.1998 Dec 15; 180
AB  - Insertion of an 18-amino-acid-encoding sequence within thefpvA gene identified permissive sites at residues Y350, A402, R451, R521, and R558, consistent with these residues occurring in extramembranous loop regions of the protein. Insertions at R451, R521, and R558 did not adversely affect receptor function, although insertions at Y350 and A402 compromised ferric pyoverdine binding and uptake. The latter region likely contributes to or interacts with the ligand-binding site.