Table 1.

Bacterial strains and plasmids used

Strain or plasmidRelevant characteristicsReference or source
E. coli strains
 TG1supEΔ(lac-proAB) thi hsdRΔ5 (F′ traD36 proA+B+ lacIqZΔM15)26
 pop2136endA thi hsdR malT::cI857O. Raibaud, Institut Pasteur
P. aeruginosa strains
 PAO1Prototroph, chl-2Holloway collection
 DZQ40PAO1 with xcpP to -Z chromosomal deletionThis study
 KS910-503xcpY51 met-901147
Plasmids
 pUC19ColE1 replicon, lacI φ80dlacZApr49
 pYZ4ColE1 replicon,oriF1 placUV5Kmr50
 pRK2013ColE1 replicon, Tra+ Mob+ (RK2) Kmr13
 pLAFR3pLAFR1 derivative, IncP1 oriT cosTcr16
 pMMB67HERSF1010 replicon, ptac lacIqApr17
 pMMB190RSF1010 replicon, ptac placUV5lacIq, Apr29
 pAX24pLAFR3 with xcpP to -Z on 20-kb DNA insert15
 pLFR41.8-kbPstI-AsuII DNA insert carrying xcpRcloned in pLAFR3This study
 pMR1Same insert as pLFR4 cloned in pMMB67HEThis study
 pSB311.4-kbSalI-SphI insert carrying xcpY cloned in pMMB67HEThis study
 pSB341.35-kbSalI-PstI insert carrying xcpY cloned in pYZ4This study
 pMYSxcpY′ (Met1→Pro234) cloned in pMMB190This study
 pSB72lasBSP-′xcpY(Arg259→stop) cloned in pMMB19028
 pMYR1.35-kb SalI-PstI and 1.8-kbPstI-AsuII DNA inserts carrying xcpYand xcpR cloned together in pMMB67HEThis study