TABLE 3

Equilibrium binding constantsa for PrrF1, PrrF2, Hfq, and antR

ComplexKd1 (nM)Kd2 (nM)
PrrF1 + Hfq65 ± 7, 51 ± 2135 ± 12, 183 ± 407
PrrF2 + Hfq70 ± 2, 74 ± 3207 ± 7, 480 ± 50
antR + Hfq295 ± 9, 302 ± 24440 ± 500, 407 ± 943
PrrF1 + antR2.5 ± 1, 7 ± 1NDb
  • a Equilibrium binding constants were determined from EMSA data as described in Materials and Methods. Shown are the values ± the standard error of the fit parameter for each individual experiment (n = 2).

  • b ND, no second PrrF1-to-antR binding event was detected (Fig. 6A and B).