TABLE 2

PrrF1 and PrrF2 are redundant in their regulation of AQ production

StrainNormalized concn [(μg/μl)/OD600]a
C7-PQSC9-PQSHHQNHQHQNONQNO
Wild type0.64 ± 0.061.01 ± 0.100.08 ± 0.020.27 ± 0.072.39 ± 0.2812.21 ± 1.03
ΔprrF1 mutant0.54 ± 0.080.65 ± 0.05d0.05 ± 0.010.14 ± 0.03b2.06 ± 0.629.82 ± 0.01
ΔprrF2 mutant0.55 ± 0.070.80 ± 0.06c0.06 ± 0.010.17 ± 0.052.50 ± 0.6111.10 ± 1.99
ΔprrF1,2 mutant0.50 ± 0.07b0.35 ± 0.06d0.04 ± 0.01b0.06 ± 0.01d0.95 ± 0.27d3.71 ± 0.00d
  • a The concentration of each AQ in the supernatants of the indicated strains grown in DTSB was determined by LC–MS-MS and normalized by culture density, as described in Materials and Methods.

  • b P < 0.05, comparing the ΔprrF1, ΔprrF2, or ΔprrF1,2 mutant to the wild type.

  • c P < 0.005, comparing the ΔprrF1, ΔprrF2, or ΔprrF1,2 mutant to the wild type.

  • d P < 0.0005, comparing the ΔprrF1, ΔprrF2, or ΔprrF1,2 mutant to the wild type.