TABLE 1

Strains used in this study

StrainGenotypeSource or reference
E. coli
    Omni MaxF′ [proAB lacIq lacZΔM15 Tn10(Tetr) Δ(ccdAB)] mcrA Δ(mrr hsdRMS-mcrBC) ϕ80(lacZ)ΔM15 Δ(lacZYA-arg*F)U169 endA1 rec*A1 supE44 thi-1 gyr*A96 relA1 ton*A panDInvitrogen
    BL21 RosettaF ompT hsdSB(rB mB) gal dcm (DE3) pRARE (Camr)Invitrogen
    BL21λDE3fhuA2 [lon] ompT gal (λ DE3) [dcm] ΔhsdS λDE3 [sBamHIo ΔEcoRI-B int::(DE3) (lacI::PlacUV5::T7 gene 1) i21 Δnin5]Invitrogen
    ANC108BL21λDE3/pAC108 (pET21b 6×His-rsiV+)
    ANC128BL21λDE3/pAC115 (pET21b 6×His-rsiVA66W)
    ANC114BL21λDE3/pAC112 (pET21 6×His-sipS38-184)
    ANC220BL21λDE3/pAC110 (pET21 6×His-rtev-sipS)
    ANC232BL21λDE3/pAC113 (pET21 6×His-rtev-sipSS43A)
    ANC258BL21λDE3/pCE602 (pET21 6×His-rtev-sipU)
    ANC259BL21λDE3/pCE601 (pET21 6×His-rtev-sipT)
    ANC260BL21λDE3/pCE603 (pET21 6×His-rtev-sipV)
B. subtilis
    PY79Prototrophic derivative of B. subtilis 16861
    CDE2373ΔsigV::Phs-rsiV+ (kan)
    LTL380ΔsigV::Phs-rsiV+ (kan) thrC::lacI+ (erm)
    LTL381ΔsigV::Phs-rsiV+ (kan) ΔsipT::spec thrC::lacI+ (erm)
    LTL412ΔsigV::Phs-rsiV+ (kan) ΔsipSR84H thrC::lacI+ (erm)
    LTL413ΔsigV::Phs-rsiV+ (kan) ΔsipS thrC::lacI+ (erm)
    LTL414ΔsigV::Phs-rsiV+ (kan) ΔsipSR84H sipT::spec thrC::lacI+ (erm)
    JLH858ΔsipT::spec
    BKE23310ΔsipS::erm62